Rizwan Muhammad, Genasan Krishnamurithy, Murali Malliga Raman, Balaji Raghavendran Hanumantha Rao, Alias Rodianah, Cheok Yi Ying, Wong Won Fen, Mansor Azura, Hamdi M, Basirun Wan Jeffrey, Kamarul Tunku
Department of Metallurgical Engineering, Faculty of Chemical and Process Engineering, NED University of Engineering and Technology 75270 Karachi Pakistan
Centre of Advanced Manufacturing and Material Processing, University of Malaya 50603 Kuala Lumpur Malaysia
RSC Adv. 2020 Jun 23;10(40):23813-23828. doi: 10.1039/d0ra04227g. eCollection 2020 Jun 19.
The low-pressure spark plasma sintering (SPS) technique is adopted to fabricate hydroxyapatite-bioglass (HA-BG) scaffolds while maintaining the physical properties of both components, including their bulk and relative density and hardness. However, prior to their orthopaedic and dental applications, these scaffolds must be validated pre-clinical assessments. In the present study, scaffolds with different ratios of HA : BG, namely, 100 : 0 (HB 0 S), 90 : 10 (HB 10 S), 80 : 20 (HB 20 S) and 70 : 30 (HB 30 S) were fabricated. These scaffolds were characterized by investigating their physicochemical properties (X-ray diffraction (XRD) and surface wettability), bioactivity in a simulated body fluid (SBF) (field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR) and calcium dissolution), antimicrobial properties, biocompatibility and osteoinduction of human bone marrow-derived mesenchymal stromal cells (hBMSCs) and human monocyte immune cell response. The XRD and surface wettability results confirmed no formation of undesirable phases and the enhanced surface hydrophilicity of the scaffolds, respectively. The bioactivity in SBF indicated the formation of bone-like apatite on the surface of the scaffolds, corresponding to an increase in BG%, which was confirmed through FTIR spectra and the increasing trend of calcium release in SBF. The scaffolds showed inhibition properties against and . The scanning electron microscopy (SEM) micrographs and Alamar Blue proliferation assay indicated the good attachment and significant proliferation, respectively, of hBMSCs on the scaffolds. Alizarin Red S staining confirmed that the scaffolds supported the mineralisation of hBMSCs. The osteogenic protein secretion (bone morphogenetic protein-2 (BMP2), type-I collagen (COL1) and osterix (OSX)) was significant on the HB 30 S-seeded hBMSCs when compared with that of HB 0 S. The monocyte migration was significantly halted in response to HA-BG-conditioned media when compared with the positive control (monocyte chemoattractant protein-1: MCP-1). In conclusion, the HB 30 S composite scaffold has a greater potential to substitute bone grafts in orthopaedic and dental applications.
采用低压放电等离子体烧结（SPS）技术制备羟基磷灰石-生物玻璃（HA-BG）支架，同时保持两种组分的物理性能，包括它们的体积、相对密度和硬度。然而，在其骨科和牙科应用之前，这些支架必须经过临床前评估验证。在本研究中，制备了具有不同HA : BG比例的支架，即100 : 0（HB 0 S）、90 : 10（HB 10 S）、80 : 20（HB 20 S）和70 : 30（HB 30 S）。通过研究这些支架的物理化学性质（X射线衍射（XRD）和表面润湿性）、在模拟体液（SBF）中的生物活性（场发射扫描电子显微镜（FESEM）、傅里叶变换红外光谱（FTIR）和钙溶解）、抗菌性能、生物相容性以及人骨髓间充质基质细胞（hBMSCs）的成骨诱导作用和人单核细胞免疫细胞反应对其进行了表征。XRD和表面润湿性结果分别证实了未形成不良相且支架的表面亲水性增强。SBF中的生物活性表明在支架表面形成了类骨磷灰石，这与BG%的增加相对应，通过FTIR光谱和SBF中钙释放的增加趋势得到了证实。这些支架对[此处原文缺失两种被抑制的对象]显示出抑制特性。扫描电子显微镜（SEM）照片和Alamar Blue增殖试验分别表明hBMSCs在支架上的良好附着和显著增殖。茜素红S染色证实支架支持hBMSCs的矿化。与HB 0 S相比，在接种HB 30 S的hBMSCs上成骨蛋白分泌（骨形态发生蛋白-2（BMP2）、I型胶原蛋白（COL1）和osterix（OSX））显著。与阳性对照（单核细胞趋化蛋白-1：MCP-1）相比，单核细胞迁移在对HA-BG条件培养基的反应中显著停止。总之，HB 30 S复合支架在骨科和牙科应用中具有更大的替代骨移植的潜力。
