Kim Yong Ook, Park Hae Woong, Kim Jong Hoon, Lee Jae Young, Moon Seong Hoon, Shin Chul Soo
Department of Biotechnology, College of Engineering, Yonsei University, Shinchon-dong, Seodaemoon-gu, Seoul 120-749, South Korea.
Life Sci. 2006 May 30;79(1):72-80. doi: 10.1016/j.lfs.2005.12.047. Epub 2006 Feb 3.
The endo-polysaccharide extracted from mycelia of Inonotus obliquus (Pers.:Fr.) Pil. (Hymenochaetaceae) is a specific activator of B cells and macrophages. However, the in vivo anti-cancer effects and the chemical structure of the endo-polysaccharide are unknown. We purified the endo-polysaccharide, investigated its anti-cancer effects via in vitro and in vivo assays, and performed a structural characterization. The endo-polysaccharide was extracted from I. obliquus mycelia cultivated in a 300-l pilot fermenter, followed by hot water extraction and ethanol precipitation. Purification was achieved by DEAE-cellulose ion-exchange chromatography and gel-permeation chromatography. Chemical analysis revealed that the purified endo-polysaccharide is an alpha-linked fucoglucomannan with a molecular weight of approximately 1,000 kDa. The anti-cancer activities of the endo-polysaccharide against various types of tumor cells were determined. No direct toxicity against either cancer or normal cells was observed. Intraperitoneal administration of the endo-polysaccharide significantly prolonged the survival rate of B16F10-implanted mice, resulting in a 4.07-fold increase in the survival rate at a dose of 30 mg/kg/day. After 60 days of feeding, approximately 67% of the initial number of mice survived with no tumor incidence based on macroscopic examination. These results indicate that the anti-cancer effect of endo-polysaccharide is not directly tumorcidal but rather is immuno-stimulating.
从桦褐孔菌（Inonotus obliquus (Pers.:Fr.) Pil.，刺革菌科）菌丝体中提取的胞内多糖是B细胞和巨噬细胞的特异性激活剂。然而，该胞内多糖的体内抗癌作用及其化学结构尚不清楚。我们对该胞内多糖进行了纯化，通过体外和体内试验研究了其抗癌作用，并进行了结构表征。该胞内多糖是从在300升中试发酵罐中培养的桦褐孔菌菌丝体中提取的，随后进行热水提取和乙醇沉淀。通过DEAE-纤维素离子交换色谱和凝胶渗透色谱实现纯化。化学分析表明，纯化后的胞内多糖是一种α-连接的岩藻糖葡甘露聚糖，分子量约为1000 kDa。测定了该胞内多糖对各种类型肿瘤细胞的抗癌活性。未观察到对癌细胞或正常细胞的直接毒性。腹腔注射该胞内多糖可显著延长接种B16F10的小鼠的存活率，在剂量为30 mg/kg/天时，存活率提高了4.07倍。喂食60天后，根据肉眼检查，约67%的初始小鼠存活，无肿瘤发生。这些结果表明，胞内多糖的抗癌作用不是直接杀肿瘤，而是免疫刺激作用。
