Olennikov Daniil N, Gornostai Tatyana G
Laboratory of Biomedical Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Sakh'yanovoy Street 6, 670047 Ulan-Ude, Russia.
Laboratory of Plant Physiological Genetics, Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Science, Lermontova Street 132, 664033 Irkutsk, Russia.
Polymers (Basel). 2023 Mar 1;15(5):1257. doi: 10.3390/polym15051257.
is a small genus of xylotrophic basidiomycetes and a source of bioactive fungochemicals among which a special place is occupied by polymeric compounds. In this study, polysaccharides that are widespread in Europe, Asia, and North America and a poorly understood fungal species, (Pers.) Karst. (fox polypore), were investigated. Water-soluble polysaccharides of mycelium were extracted, purified, and studied using chemical reactions, elemental and monosaccharide analysis, UV-Vis and FTIR spectroscopy, gel permeation chromatography, and linkage analysis. Five homogenic polymers (IRP-1-IRP-5) with molecular weights of 110-1520 kDa were heteropolysaccharides that consist mainly of galactose, glucose, and mannose. The dominant component, IRP-4, was preliminary concluded to be a branched (1→3,6)-linked galactan. Polysaccharides of inhibited the hemolysis of sensitized sheep erythrocytes by complement from human serum, signifying anticomplementary activity with the greatest effects for the IRP-4 polymer. These findings suggest that mycelium is a new source of fungal polysaccharides with potential immunomodulatory and anti-inflammatory properties.
是木生担子菌的一个小属，也是生物活性真菌化学物质的一个来源，其中聚合物化合物占有特殊地位。在本研究中，对在欧洲、亚洲和北美广泛分布且鲜为人知的真菌物种——（Pers.）Karst.（狐多孔菌）的多糖进行了研究。提取、纯化了狐多孔菌菌丝体的水溶性多糖，并通过化学反应、元素和单糖分析、紫外可见光谱和傅里叶变换红外光谱、凝胶渗透色谱以及键合分析对其进行了研究。五种分子量为110 - 1520 kDa的同质性聚合物（IRP - 1 - IRP - 5）是杂多糖，主要由半乳糖、葡萄糖和甘露糖组成。主要成分IRP - 4初步推断为一种分支的（1→3,6）连接的半乳聚糖。狐多孔菌的多糖抑制了人血清补体对致敏绵羊红细胞的溶血作用，表明具有抗补体活性，其中IRP - 4聚合物的效果最为显著。这些发现表明，狐多孔菌菌丝体是具有潜在免疫调节和抗炎特性的真菌多糖的新来源。
