Chen Chin-Chu, Liu Yi-Wen, Ker Yaw-Bee, Wu Yen-Yin, Lai Eric Y, Chyau Charng-Cherng, Hseu Tzong-Hsiung, Peng Robert Y
Institute of Biotechnology, National Tsing Hua University, No. 101, Section 2 Kuang-Fu Road, Hsinchu, Taiwan, Republic of China.
J Agric Food Chem. 2007 Jun 27;55(13):5007-12. doi: 10.1021/jf063484c. Epub 2007 May 31.
Five polysaccharide fractions (AC-1, AC-2, AC-3, AC-4, and AC-5) were obtained after systemic solvent extractions and precipitations from Antrodia camphorata mycelia with yields of 2.92, 10.38, 1.65, 0.34, and 1.64%, respectively. Gel permeation chromatography (GPC) analysis showed that the distribution of mean molecular mass of the fractionated polysaccharides was in the range of 394-940 kDa. The proximate compositions from each polysaccharide fraction revealed that all fractions belonged to the category of glycoprotein, having ratios of carbohydrate/protein ranging from 0.29 to 10.79 (w/w). Glucose or galactose was the major monosaccharide in all fractions except fraction AC-2, which has a mean molecular mass of 394 kDa with lyxose as the most prominent constituent. In the evaluation of the DPPH* radical scavenging capability, fraction AC-1 and AC-2 polysaccharides showed the better capabilities, around 74.5 and 50.5%, respectively, compared to the reference control of Trolox (87.5%) at a concentration of 1 microM. In testing with macrophage RAW264.7 cells, fraction AC-2 demonstrated a rather potent anti-inflammatory capability. Furthermore, the lipopolysaccharide-induced NO production and the protein expression by the inducible nitric oxide synthase (iNOS) gene were inhibited, respectively, in a dose-dependent (50-200 microg/mL) manner by fraction AC-2 polysaccharide.
通过对樟芝菌丝体进行系统的溶剂萃取和沉淀，获得了五个多糖级分（AC - 1、AC - 2、AC - 3、AC - 4和AC - 5），产率分别为2.92%、10.38%、1.65%、0.34%和1.64%。凝胶渗透色谱（GPC）分析表明，分级多糖的平均分子量分布在394 - 940 kDa范围内。每个多糖级分的近似组成显示，所有级分均属于糖蛋白类别，碳水化合物/蛋白质的比例在0.29至10.79（w/w）之间。除AC - 2级分（平均分子量为394 kDa，以来苏糖为最主要成分）外，葡萄糖或半乳糖是所有级分中的主要单糖。在评估DPPH*自由基清除能力时，AC - 1和AC - 2级分多糖表现出较好的清除能力，在1 microM浓度下，分别约为74.5%和50.5%，而对照品Trolox的清除率为87.5%。在用巨噬细胞RAW264.7细胞进行测试时，AC - 2级分表现出相当强的抗炎能力。此外，AC - 2级分多糖以剂量依赖性（50 - 200 microg/mL）方式分别抑制了脂多糖诱导的NO产生和诱导型一氧化氮合酶（iNOS）基因的蛋白表达。
