Ramji D P, Donovan J T, Hames B D
Department of Biochemistry and Molecular Biology, University of Leeds, UK.
Mol Microbiol. 1991 Feb;5(2):427-32. doi: 10.1111/j.1365-2958.1991.tb02125.x.
The 7E gene is expressed late in normal development of Dictyostelium discoideum after pseudoplasmodium formation. After disaggregation of the developing cells, transcription of this gene depends entirely on exogenous 3'5' cyclic AMP (cAMP). The 5' flanking region of the 7E gene contains two TATA box-oligo (dT) promoter motifs but analysis of 7E gene expression by primer extension shows only a single primary transcript with transcription initiating immediately after the most proximal promoter motif during development or in disaggregated cells in the presence of exogenous cAMP. Four C-rich sequences lie within 350bp upstream of the cap site, analogous to the upstream elements implicated in the cAMP regulation of several other Dictyostelium genes expressed in development.
7E基因在盘基网柄菌假原质团形成后的正常发育后期表达。发育中的细胞解体后，该基因的转录完全依赖于外源性3',5'-环磷酸腺苷（cAMP）。7E基因的5'侧翼区域包含两个TATA盒-寡聚（dT）启动子基序，但通过引物延伸分析7E基因表达显示，在发育过程中或存在外源性cAMP的解体细胞中，只有一个主要转录本，转录在最近端启动子基序之后立即开始。四个富含C的序列位于帽位点上游350bp内，类似于与发育中表达的其他几个盘基网柄菌基因的cAMP调节有关的上游元件。
