Ramji D P, Richards A J, Jagger P, Bleasby A, Hames B D
Department of Biochemistry, University of Leeds, UK.
Mol Microbiol. 1990 Jan;4(1):129-35. doi: 10.1111/j.1365-2958.1990.tb02022.x.
Expression of the 7E and 2C genes late in Dictyostelium development ceases upon cell disaggregation but, in contrast to many other genes we have studied, expression is fully restored by exogenous cAMP (A. J. Richards et al., submitted). The 7E and 2C genes encode polypeptides of similar size (9220 and 10573 Daltons, respectively), each of which contains an unusually high proportion of serine plus glycine residues (41% and 59%, respectively). Each protein possesses a relatively serine-rich N-terminus and glycine-rich C-terminus and contains the conserved sequence S(X)SSS(X2)SS(X)SS(X2)SFGS. These data suggest that genes 7E and 2C may have arisen by duplication of a common ancestor. Computer analysis indicates that both gene products are probably intracellular structural proteins that form extended coil structures.
盘基网柄菌发育后期7E和2C基因的表达在细胞解体时停止，但与我们研究过的许多其他基因不同，外源性环磷酸腺苷（cAMP）可使其表达完全恢复（A. J. 理查兹等人，待发表）。7E和2C基因编码大小相似的多肽（分别为9220和10573道尔顿），每个多肽都含有异常高比例的丝氨酸加甘氨酸残基（分别为41%和59%）。每种蛋白质都有一个相对富含丝氨酸的N端和富含甘氨酸的C端，并包含保守序列S(X)SSS(X2)SS(X)SS(X2)SFGS。这些数据表明，7E和2C基因可能是由一个共同祖先基因复制而来。计算机分析表明，这两种基因产物可能都是形成延伸螺旋结构的细胞内结构蛋白。
