Razin S V, Vassetzky Y S, Hancock R
Institute of Gene Biology, Academy of Sciences of the USSR, Moscow.
Biochem Biophys Res Commun. 1991 May 31;177(1):265-70. doi: 10.1016/0006-291x(91)91977-k.
We have mapped nuclear matrix attachment regions (MARs), defined by their specific binding to nuclear matrices in vitro, and sites of topoisomerase II reaction, detected by DNA cleavage in vitro in the presence of the inhibitor VM-26, in the vicinity of the replication origin of the chicken alpha-globin gene domain. Two MARs are located close to the downstream end (in the direction of transcription) of a 3 kb fragment which includes the origin. These MARs contain sites for strong topoisomerase II binding and reaction. Our observations on this gene domain support two hypotheses concerning MARs in eukaryotic cells, namely that they are close to DNA replication origins and that they contain multiple topoisomerase II recognition sites.
我们已绘制了核基质附着区域（MARs），其通过体外与核基质的特异性结合来定义，以及拓扑异构酶II反应位点，该位点通过在抑制剂VM - 26存在下的体外DNA切割来检测，这些都位于鸡α - 珠蛋白基因结构域复制起点附近。两个MARs位于一个包含该起点的3 kb片段的下游末端（转录方向）附近。这些MARs含有强拓扑异构酶II结合和反应的位点。我们对该基因结构域的观察结果支持了关于真核细胞中MARs的两个假说，即它们靠近DNA复制起点且含有多个拓扑异构酶II识别位点。
