Yeagle P L, Epand R M, Richardson C D, Flanagan T D
Department of Biochemistry, University at Buffalo, School of Medicine and Biomedical Sciences, NY 14214.
Biochim Biophys Acta. 1991 May 31;1065(1):49-53. doi: 10.1016/0005-2736(91)90009-w.
31P nuclear magnetic resonance spectroscopy (31P-NMR) was used to study phospholipid organization in hydrated preparations of N-methyl dioleoylphosphatidylethanolamine and a 'fusion peptide' with the sequence: FAGV-VLAGAALGVAAAAQI, which corresponds to the amino terminus of the F1 subunit of the membrane fusion protein of measles virus. These amino acids are believed to mediate syncytia formation, host-cell penetration and hemolysis by infectious virus. The presence of the peptide at 0.5 mole percent significantly facilitated the formation of isotropic 31P resonances. The effects at 1 mole percent peptide were substantially enhanced over the effects observed at 0.5 mole percent, leading to a decrease in the onset temperature of the formation of the isotropic 31P-NMR resonances by about 30 degrees C. The formation of such isotropic 31P-NMR resonances has been previously associated with an increased rate of fusion of large unilamellar vesicles composed of N-methyl dioleoylphosphatidylethanolamine. Enhanced fusion of octadecyl rhodamine-labelled Sendai virus with N-methyl dioleoylphosphatidylethanolamine large unilamellar vesicles was observed when the 'fusion peptide' was incorporated into the large unilamellar vesicles.
采用³¹P核磁共振波谱法（³¹P-NMR）研究了N-甲基二油酰磷脂酰乙醇胺水合制剂以及一种“融合肽”（序列为：FAGV-VLAGAALGVAAAAQI，对应麻疹病毒膜融合蛋白F1亚基的氨基末端）中的磷脂组织。据信这些氨基酸介导了感染性病毒的多核体形成、宿主细胞穿透和溶血过程。该肽以0.5摩尔百分比存在时显著促进了各向同性³¹P共振的形成。1摩尔百分比肽的效果比0.5摩尔百分比时观察到的效果大幅增强，导致各向同性³¹P-NMR共振形成的起始温度降低约30℃。此前已发现这种各向同性³¹P-NMR共振的形成与由N-甲基二油酰磷脂酰乙醇胺组成的大单层囊泡融合速率增加有关。当将“融合肽”掺入大单层囊泡时，观察到十八烷基罗丹明标记的仙台病毒与N-甲基二油酰磷脂酰乙醇胺大单层囊泡的融合增强。
