Yeagle P L, Smith F T, Young J E, Flanagan T D
Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14214.
Biochemistry. 1994 Feb 22;33(7):1820-7. doi: 10.1021/bi00173a027.
The ability of lysophosphatidylcholine to inhibit membrane fusion at subsolubilizing concentrations (between 1 and 9 mol % with respect to the membrane lipids) was examined. Fusion between N-methyldioleoylphosphatidylethanolamine (DOPE) large unilamellar vesicles (LUV) and fusion between Sendai virus and N-methyl-DOPE LUV were measured. A contents mixing fusion assay was used for LUV fusion (ANTS/DPX), and a lipid mixing assay (octadecylrhodamine B) was used for the virus fusion experiments. Lysophosphatidylcholine was effective at inhibiting both LUV fusion and Sendai virus/LUV fusion. Lysophosphatidylcholine also inhibited leakage from N-methyl-DOPE LUV, 31P nuclear magnetic resonance data were obtained of N-methyl-DOPE in the presence of lysophosphatidylcholine. Lysophosphatidylcholine stabilized the lamellar phase and reduced the incidence of nonlamellar structures at all temperatures. The destabilization of nonlamellar structures with a negative radius of curvature may be a mechanism for inhibition of fusion by lysophosphatidylcholine in these systems.
研究了溶血磷脂酰胆碱在亚溶解浓度（相对于膜脂为1至9摩尔%）下抑制膜融合的能力。测量了N-甲基二油酰磷脂酰乙醇胺（DOPE）大单层囊泡（LUV）之间的融合以及仙台病毒与N-甲基-DOPE LUV之间的融合。对于LUV融合（ANTS/DPX）使用了内容物混合融合测定法，对于病毒融合实验使用了脂质混合测定法（十八烷基罗丹明B）。溶血磷脂酰胆碱在抑制LUV融合和仙台病毒/LUV融合方面均有效。溶血磷脂酰胆碱还抑制了N-甲基-DOPE LUV的渗漏，获得了存在溶血磷脂酰胆碱时N-甲基-DOPE的31P核磁共振数据。溶血磷脂酰胆碱在所有温度下均稳定层状相并降低了非层状结构的发生率。具有负曲率半径的非层状结构的去稳定化可能是这些系统中溶血磷脂酰胆碱抑制融合的一种机制。
