Aguilar L V, Lazcano-Ponce E, Vaccarella S, Cruz A, Hernández P, Smith J S, Muñoz N, Kornegay J R, Hernández-Avila M, Franceschi S
Escuela Militar de Graduados de Sanidad, Universidad del Ejército y Fuerza Aérea Mexicanos, Mexico DF, Mexico.
Sex Transm Infect. 2006 Feb;82(1):31-3. doi: 10.1136/sti.2005.015131.
To elucidate which anatomical sites need to be sampled to detect human papillomavirus (HPV) infection in the lower male genital tract.
In an HPV survey of Mexican soldiers (median age 24 years; range 16-50 years), a cell sample from 2 cm deep into the distal urethra (group 1; n = 168 men), or 0.5 cm deep into the meatus urethralis (group 2; n = 414 men) was collected, along with a sample from the external genitalia. The different samples were tested for 27 HPV types using a polymerase chain reaction based strip assay.
HPV DNA was detected more frequently in external genitalia samples (46.4%) than in the urethra (20.8%) or meatus samples (12.1%). Lack of samples from the urethra or meatus would have led to 5.1% and 1.5% false HPV negative results, respectively. The most frequently detected high risk HPV types (HPV 59, 52, 51, and 16) were similar in different sites, whereas low risk types were found rarely in urethra samples.
The addition of cell samples from the meatus to those from external genitalia contributed negligibly to the evaluation of the prevalence of HPV in men. HPV detection was slightly improved by the addition of urethra samples, but the gain may not justify the discomfort of the procedure in large epidemiological studies.
阐明检测男性下生殖道人乳头瘤病毒（HPV）感染时需要采集哪些解剖部位的样本。
在一项针对墨西哥士兵（中位年龄24岁；范围16 - 50岁）的HPV调查中，采集距尿道远端2 cm深处的细胞样本（第1组；n = 168名男性）或距尿道口0.5 cm深处的细胞样本（第2组；n = 414名男性），同时采集外生殖器样本。使用基于聚合酶链反应的试纸检测法对不同样本进行27种HPV类型的检测。
在外生殖器样本中检测到HPV DNA的频率（46.4%）高于尿道样本（20.8%）或尿道口样本（12.1%）。未采集尿道或尿道口样本分别会导致5.1%和1.5%的HPV假阴性结果。不同部位最常检测到的高危HPV类型（HPV 59、52、51和16）相似，而低危类型在尿道样本中很少发现。
在外生殖器样本基础上增加尿道口细胞样本对评估男性HPV感染率的贡献可忽略不计。增加尿道样本可略微提高HPV检测率，但在大型流行病学研究中，这种提高可能无法弥补该操作带来的不适。
