Claassen J A H R, Colier W N J M, Jansen R W M M
Department of Geriatric Medicine, Radboud University Medical Centre, Department of Geriatric Medicine, Nijmegen, The Netherlands.
Physiol Meas. 2006 Mar;27(3):255-64. doi: 10.1088/0967-3334/27/3/004. Epub 2006 Jan 20.
Near infrared spectroscopy (NIRS) is a non-invasive method to monitor cerebral haemodynamics. Used either alone or in combination with other non-invasive methods such as transcranial Doppler sonography, this technique is well suited for use in cerebrovascular research in ageing. Reproducibility of NIRS, however, has only been determined in neonates and adults. We applied controlled desaturation (the O(2)-method) to measure the cerebral blood volume (CBV) with NIRS in 16 healthy subjects aged 65 to 88. This method uses deoxygenated haemoglobin (the concentration of which is manipulated by desaturation) as an intravascular tracer for NIRS. We determined repeatability (between tests interval: 2 min), short-term reproducibility (intervals of 20 and 40 min) and long-term reproducibility (interval > 2 weeks). We found a coefficient of variation (CV) of 12.5% for repeatability and a CV of 11.7% for short-term reproducibility. The CV for long-term reproducibility was 15%. We conclude that NIRS can reproducibly measure CBV in subjects aged 65 and older, using the O(2)-method. In this group of healthy subjects, this method was well tolerated.
近红外光谱法（NIRS）是一种监测脑血流动力学的非侵入性方法。该技术单独使用或与其他非侵入性方法（如经颅多普勒超声检查）联合使用时，非常适合用于衰老方面的脑血管研究。然而，NIRS的可重复性仅在新生儿和成年人中得到了确定。我们应用控制性去饱和（O₂法），通过NIRS对16名年龄在65至88岁的健康受试者的脑血容量（CBV）进行测量。该方法使用脱氧血红蛋白（其浓度通过去饱和来控制）作为NIRS的血管内示踪剂。我们测定了重复性（测试间隔：2分钟）、短期可重复性（间隔20分钟和40分钟）和长期可重复性（间隔>2周）。我们发现重复性的变异系数（CV）为12.5%，短期可重复性的CV为11.7%。长期可重复性的CV为15%。我们得出结论，使用O₂法，NIRS能够可重复地测量65岁及以上受试者的CBV。在这组健康受试者中，该方法耐受性良好。
