Tsutsui Y, Yamazaki Y
Institute for Developmental Research, Aichi Prefectural Colony, Japan.
J Gen Virol. 1991 Jun;72 ( Pt 6):1415-9. doi: 10.1099/0022-1317-72-6-1415.
The 72K immediate early (IE) 1 protein of human cytomegalovirus and the 68K protein, also encoded by the IE1 gene, were detected by immunoelectron microscopy using monoclonal antibodies specific for the 68K or 72K proteins. Early after infection, both proteins were localized to the nucleus, but with different localization patterns. Late after infection, both of the proteins decreased markedly in the nucleus, in which nucleocapsids appeared. Simultaneously, the 68K protein became diffusely distributed in the cytoplasm and the plasma membrane, whereas the 72K protein was distributed in the nuclear envelope and the plasma membrane. Both proteins were also observed in the coating membrane of the extracellular dense bodies.
利用针对68K或72K蛋白的单克隆抗体，通过免疫电子显微镜检测了人巨细胞病毒的72K即刻早期（IE）1蛋白以及同样由IE1基因编码的68K蛋白。感染后早期，这两种蛋白均定位于细胞核，但定位模式不同。感染后期，这两种蛋白在细胞核中的含量显著降低，细胞核中出现核衣壳。同时，68K蛋白开始弥散分布于细胞质和质膜中，而72K蛋白则分布于核膜和质膜中。在细胞外致密体的包膜中也观察到了这两种蛋白。
