Rodgers B, Borysiewicz L, Mundin J, Graham S, Sissons P
Department of Medicine, Royal Postgraduate Medical School, London, U.K.
J Gen Virol. 1987 Sep;68 ( Pt 9):2371-8. doi: 10.1099/0022-1317-68-9-2371.
An early antigen of human cytomegalovirus (HCMV) was purified from infected cells as a 72K polypeptide by immunoaffinity chromatography using a monoclonal antibody. It was located in both nucleus and cytoplasm, and was non-glycosylated and undetectable on the surface of infected cells. Known seropositive subjects had antibody against the purified protein and it elicited proliferative T cell responses in 10 of 16 subjects. Five of 14 T cell lines established in response to the purified protein were predominantly CD8+ and of these two showed major histocompatibility complex-restricted cytotoxicity against HCMV-infected cells. This provides further evidence that antigens expressed at early times may be targets for the immune response during persistent HCMV infection.
用人巨细胞病毒（HCMV）的一种早期抗原，通过使用单克隆抗体的免疫亲和层析法，从受感染细胞中纯化出一种72K多肽。它定位于细胞核和细胞质中，非糖基化，在受感染细胞表面无法检测到。已知血清反应阳性的受试者体内有针对该纯化蛋白的抗体，并且在16名受试者中有10名产生了增殖性T细胞反应。针对该纯化蛋白建立的14个T细胞系中，有5个主要为CD8 +，其中2个表现出针对HCMV感染细胞的主要组织相容性复合体限制的细胞毒性。这进一步证明，在持续性HCMV感染期间，早期表达的抗原可能是免疫反应的靶标。
