人疱疹病毒性脑炎家兔脑脊液中的胸苷激酶活性

Thymidine kinase activity in cerebrospinal fluid of rabbits with Herpesvirus hominis encephalitis.

作者信息

Hilty M D, Thomas D C, Haynes R E, Cramblett H G

出版信息

Pediatr Res. 1975 Mar;9(3):143-6. doi: 10.1203/00006450-197503000-00007.

DOI:10.1203/00006450-197503000-00007

Abstract

The rabbit model of Herpesvirus hominis (HVH) encephalitis was utilized to determine whether thymidine (TdR) kinase (EC. 2.7.1.2.1), AN EARLY ENZYME IN THe HVH replicative cycle, is present in the cerebrospinal fluid (CSF) of rabbits with HVH encephalitis. The mean TdR kinase activity in CSF from infected rabbits was 2.30 plus or minus 1.69 pmol product/120 min/100 mul CSF, while the mean TdR kinase activity of uninfected CSF was 0.34 plus or minus 0.25. The enzyme appeared to be cell associated since the TdR kinase activity in the sedimented cell suspension was 5.08 plus or minus 2.93 (seven values) while the supernatant activity was 0.14 plus or minus 0.31 (seven values). To allow a comparison of TdR kinase from infected rabbit CSF with HVH and rabbit mononuclear cell TdR kinase, the mean ratio of TdR kinase activity at pH 6.0/pH 8.0, for infected rabbit CSF is intermediate between the ratio for HVH TdR kinase (P less than 0.001) and rabbit mononuclear cell TdR kinase (P less than 0.05).

摘要

利用人疱疹病毒（HVH）脑炎的兔模型来确定胸苷（TdR）激酶（EC. 2.7.1.2.1，HVH复制周期中的一种早期酶）是否存在于患有HVH脑炎的兔的脑脊液（CSF）中。感染兔脑脊液中TdR激酶的平均活性为2.30±1.69 pmol产物/120分钟/100μl脑脊液，而未感染脑脊液的平均TdR激酶活性为0.34±0.25。该酶似乎与细胞相关，因为沉淀细胞悬液中的TdR激酶活性为5.08±2.93（七个值），而上清液活性为0.14±0.31（七个值）。为了比较感染兔脑脊液中的TdR激酶与HVH和兔单核细胞TdR激酶，感染兔脑脊液在pH 6.0/pH 8.0时TdR激酶活性的平均比值介于HVH TdR激酶的比值（P<0.001）和兔单核细胞TdR激酶的比值（P<0.05）之间。