佛波酯和鞘氨醇对人淋巴母细胞样B细胞系的调节作用。一项31P核磁共振研究。

Modulation of human lymphoblastoid B cell line by phorbol ester and sphingosine. A 31P-NMR study.

作者信息

Miccheli A, Ricciolini R, Piccolella E, Delfini M, Conti F

机构信息

Department of Chemistry, University La Sapienza, Rome, Italy.

出版信息

Biochim Biophys Acta. 1991 Jun 7;1093(1):29-35. doi: 10.1016/0167-4889(91)90134-j.

DOI:10.1016/0167-4889(91)90134-j

PMID:1646646

Abstract

Changes in phospholipid and energy metabolism in Epstein-Barr Virus transformed B lymphocytes (EBV-B), induced by phorbol 12,13-dibutyrate (PD) and sphingosine (an inhibitor of protein kinase C), have been evaluated by 31P-NMR spectroscopy. The effects of PD and sphingosine on [3H]thymidine incorporation have also been studied. An increase in phosphorylcholine (PCho) levels has been observed in sphingosine and sphingosine + PD treated cells after 30 min of incubation, whereas no change was observed in lymphocytes incubated with PD during the same period. Extracellular choline levels increased in sphingosine treated cells but decreased in PD treated cells. Hence, a sphingosine-dependent hydrolysis of choline-linked phospholipids is suggested. A time-dependent reduction of PCho observed after 120 min PD incubation is consistent with an increase of the synthesis of choline-linked phospholipids.

摘要

通过31P-核磁共振波谱法评估了佛波醇12,13-二丁酸酯（PD）和鞘氨醇（蛋白激酶C的抑制剂）诱导的爱泼斯坦-巴尔病毒转化B淋巴细胞（EBV-B）中磷脂和能量代谢的变化。还研究了PD和鞘氨醇对[3H]胸苷掺入的影响。孵育30分钟后，在经鞘氨醇和鞘氨醇+PD处理的细胞中观察到磷酸胆碱（PCho）水平升高，而同期用PD孵育的淋巴细胞中未观察到变化。鞘氨醇处理的细胞中细胞外胆碱水平升高，而PD处理的细胞中细胞外胆碱水平降低。因此，提示存在鞘氨醇依赖性的胆碱连接磷脂水解。PD孵育120分钟后观察到的PCho随时间的减少与胆碱连接磷脂合成的增加一致。