地塞米松通过鞘氨醇生成对人淋巴母细胞样B细胞系进行依赖性调节。

Dexamethasone-dependent modulation of human lymphoblastoid B cell line through sphingosine production.

作者信息

Ricciolini R, Miccheli A, Di Cocco M E, Piccolella E, Marino A, Sammartino M P, Conti F

机构信息

Department of Chemistry, University La Sapienza, Rome, Italy.

出版信息

Biochim Biophys Acta. 1994 Mar 31;1221(2):103-8. doi: 10.1016/0167-4889(94)90001-9.

DOI:10.1016/0167-4889(94)90001-9

PMID:8148386

Abstract

The relationship between dexamethasone-dependent changes in intracellular sphingosine levels, energy and phospholipid metabolism have been investigated by 31P-NMR spectroscopy and high-performance liquid chromatography. The cellular functions have been evaluated by cellular growth and immunoglobulin M secretion (IgM). Significant increases in intracellular phosphorylcholine (PCho), extracellular choline (Cho), and endogenous sphingosine levels were observed only at 30 min incubation with dexamethasone. These results confirmed a sphingosine-dependent hydrolysis of choline-linked phospholipids (Miccheli, A., Ricciolini, R., Piccolella, E., Delfini, M. and Conti, F. (1991) Biochim. Biophys. Acta 1093, 29-35). Furthermore, no significant variations were evidenced at hours 1, 2, 6 and 18 of incubation. Dexamethasone causes an inhibition of cellular growth and IgM secretion as well as the sphingosine treatment. The results suggest that the effect of dexamethasone may be mediated by endogenous sphingosine production in Epstein-Barr virus transformed B lymphocytes.

摘要

通过31P-NMR光谱法和高效液相色谱法研究了地塞米松依赖性细胞内鞘氨醇水平变化、能量和磷脂代谢之间的关系。通过细胞生长和免疫球蛋白M分泌（IgM）评估细胞功能。仅在与地塞米松孵育30分钟时，观察到细胞内磷酸胆碱（PCho）、细胞外胆碱（Cho）和内源性鞘氨醇水平显著增加。这些结果证实了胆碱连接的磷脂存在鞘氨醇依赖性水解（米凯利，A.，里乔利尼，R.，皮科莱拉，E.，德尔菲尼，M.和孔蒂，F.（1991年）《生物化学与生物物理学报》1093，29 - 35）。此外，在孵育1、2、6和18小时时未发现显著变化。地塞米松会导致细胞生长和IgM分泌受到抑制，鞘氨醇处理也是如此。结果表明，地塞米松的作用可能由爱泼斯坦 - 巴尔病毒转化的B淋巴细胞中内源性鞘氨醇的产生介导。