Boerman R H, Arnoldus E P, Peters A C, Bloem B R, Raap A K, van der Ploeg M
Department of Neurology, University Hospital Leiden, The Netherlands.
J Med Virol. 1991 Feb;33(2):83-8. doi: 10.1002/jmv.1890330204.
A series of experiments was carried out using a mouse HSV-1 encephalitis model for detecting HSV-DNA in CSF by the polymerase chain reaction (PCR). The results were correlated with the time period after corneal inoculation, clinical signs and symptoms, and progression of infection in brain tissue (antigen detection and histopathology), and were compared with virus isolation by spin-amplified culture (SAC/IF). The PCR proved to be superior to SAC/IF, both with respect to early detection and the percentage of positive samples. The results of PCR did not correlate with signs of disease, nor with the interval post-inoculation. A correlation with the spread of HSV within the brain tissue was found. The possible pathways for virus spread from brain parenchyma to the CSF as well as a possible explanation for the low amounts of virus and viral-DNA within the CSF are discussed.
利用小鼠单纯疱疹病毒1型（HSV-1）脑炎模型，通过聚合酶链反应（PCR）检测脑脊液中的HSV-DNA，进行了一系列实验。将结果与角膜接种后的时间段、临床体征和症状以及脑组织中的感染进展（抗原检测和组织病理学）相关联，并与通过旋转扩增培养（SAC/IF）进行病毒分离的结果进行比较。结果证明，在早期检测和阳性样本百分比方面，PCR均优于SAC/IF。PCR结果与疾病体征以及接种后的时间间隔均无关联。发现其与HSV在脑组织中的扩散存在相关性。文中讨论了病毒从脑实质扩散至脑脊液的可能途径，以及脑脊液中病毒和病毒DNA含量较低的可能原因。
