通过聚合酶链反应扩增脑脊液DNA的Southern印迹法诊断疱疹性脑炎。
Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction.
作者信息
Klapper P E, Cleator G M, Dennett C, Lewis A G
机构信息
Department of Pathological Sciences, University of Manchester, England.
出版信息
J Med Virol. 1990 Dec;32(4):261-4. doi: 10.1002/jmv.1890320413.
Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.
对从腰椎脑脊液（CSF）中提取的DNA进行单纯疱疹病毒胸苷激酶基因特异性聚合酶链反应（PCR）扩增以及Southern印迹法（SB），作为诊断单纯疱疹性脑炎（HSE）的一种方法进行了评估。在10例经证实的疱疹性脑炎患者中，有9例患者（12份CSF标本中的11份）的CSF样本早在神经疾病发作后2天就获得了PCR-SB阳性结果。我们的数据支持以下观点，即PCR技术可能为HSE的诊断提供一种临床相关的“非侵入性”方法。
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