[成都汉族人群与云南汉族人群5个STR基因座的复合扩增及其等位基因频率分布分析]

[Analysis of the multi-amplified 5 STR loci and their allelic distribution in both Han populations in Chengdu City and Yunnan province].

作者信息

Xing Jun-guo, Liang Wei-bo, Lü Mei-li, Du Hong, Chen Guo-di, Zhang Lin

机构信息

Key Lab of Biotherapy of Human Diseases, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):84-7.

PMID:16468650

Abstract

OBJECTIVE

To illuminate the multi-amplified 5 STR loci and their allelic distribution in Hans by means of STR-DNA typing with improved efficiency and decreased cost.

METHODS

We have established an allelic ladder of D7S820, D13S317, D5S818, D3S1358 and Amelogenin loci via the cloning techniques. With this homemade allelic ladder, we established successfully a multiplexing polymerase chain reaction (PCR) method, followed by denaturing polyacrylamide gel electrophoresis (PAGE) and silver staining. DNA samples collected from 130 unrelated Han individuals in Yunnan and Chengdu were analyzed. The non-overlapping of the allelic fragments of the five loci allowed the detection to be accomplished successfully.

RESULTS

No difference of the genotyping results of the single locus amplification and multiplexing was observed. The genotype distributions of 4 STR were in accordance with the Hardy-Weinberg equilibrium. 7, 7, 8 and 8 alleles of D7S820, D13S317, D5S818, and D3S1358 loci were observed in Yunnan Han population, as well as 8, 7, 8 and 7 alleles in Chengdu Han population respectively. No significant difference in the allele distribution of these loci was seen between these two Han populations.

CONCLUSION

This multiplexing system with home-made allelic ladder has a high combined discrimination power and exclusion power. It is a valuable tool in forensic science practice.

摘要

目的

通过提高效率和降低成本的STR-DNA分型方法，阐明汉族人群中5个STR基因座的多重扩增及其等位基因分布。

方法

通过克隆技术建立了D7S820、D13S317、D5S818、D3S1358和Amelogenin基因座的等位基因分型标准物。利用自制的等位基因分型标准物，成功建立了多重聚合酶链反应（PCR）方法，随后进行变性聚丙烯酰胺凝胶电泳（PAGE）和银染。对从云南和成都130名无血缘关系的汉族个体采集的DNA样本进行分析。五个基因座等位基因片段不重叠，使得检测得以成功完成。

结果

单基因座扩增和多重扩增的基因分型结果未观察到差异。4个STR的基因型分布符合Hardy-Weinberg平衡。在云南汉族人群中观察到D7S820、D13S317、D5S818和D3S1358基因座分别有7、7、8和8个等位基因，在成都汉族人群中分别观察到8、7、8和7个等位基因。这两个汉族人群在这些基因座的等位基因分布上没有显著差异。