Wu Yuan-Ming, Zhang Xiao-Nan, Zhou You, Chen Zhi-Yang, Wang Xiao-Bin
Department of Pathophysiology, The Fmmu Center for DNA Typing, The Fourth Military Medical University, Shaanxi Province, China.
Forensic Sci Int Genet. 2008 Mar;2(2):e15-8. doi: 10.1016/j.fsigen.2007.11.003.
Fifteen autosomal STRs loci were analyzed from a population sample of 446 unrelated healthy individuals of Chinese Han population residing in Xi'an city of Shaanxi Province using a multiplex PCR system. We report allele frequencies distribution and statistical parameters for all STR loci, D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA. The observed genotype frequencies and expected of genotype frequencies were evaluated by chi(2)-test and the Fisher exact tests. Chi-Square test showed all STR loci were in Hardy-Weinberg equilibrium (p<0.05). Our studied population data were compared with the previously published population data of other ethnics or areas. After comparing, significant differences were found between Chinese Han population in Xi'an and Korean, Tibetan, Uigur, Chinese Han population in Guangdong Province, Hui population, Chinese Salar, Dongxiang and Tu ethnic minority living in Qinghai Province of China at some loci. The forensic parameters from the data showed high values. The data in the present study can be used greatly for routine forensic application in the region of Xi'an city.
使用多重聚合酶链反应（PCR）系统，对陕西省西安市446名无亲缘关系的中国汉族健康个体的群体样本进行了15个常染色体短串联重复序列（STR）基因座分析。我们报告了所有STR基因座（D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818和FGA）的等位基因频率分布及统计参数。通过卡方检验和费舍尔精确检验评估观察到的基因型频率和预期的基因型频率。卡方检验表明所有STR基因座均处于哈迪-温伯格平衡状态（p<0.05）。我们将研究的群体数据与之前发表的其他种族或地区的群体数据进行了比较。比较后发现，西安的中国汉族群体与韩国人、藏族、维吾尔族、广东省的中国汉族群体、回族、中国撒拉族、东乡族以及居住在中国青海省的土族等少数民族在某些基因座上存在显著差异。数据得出的法医参数值较高。本研究中的数据可在西安市地区的常规法医应用中得到广泛应用。
