Polin D, Bursian S J, Underwood M S, Wiggers P A, Biondo N, Su I, Braselton W E, Render J A
Department of Animal Science, Michigan State University, East Lansing 48824.
J Toxicol Environ Health. 1991 Jun;33(2):197-212. doi: 10.1080/15287399109531518.
Rats were fed polybrominated biphenyls (PBBs) at 0.1 to 100.0 ppm for 14 d and then treated to hasten the removal of PBBs with 0, 5, or 10% mineral oil (MO) and/or 0, 15, 30, or 45% feed restriction (FR) for 21 d. PBB body burdens were determined at d 14 and expressed on a log-log basis by Y = 0.91X + 2.179 (r2 = 0.974), where X = log of PBB concentration in diet (ppm) and Y = log of PBB body burden (micrograms). After 21 d withdrawal, body burdens were expressed by the equation Y = 0.787X + 2.218 (r2 = 0.95). The most effective withdrawal treatment was 10% MO + 45% FR producing a reduction of body burdens inversely related to prior body burdens (69% at 0.1 ppm to 23% at 100 ppm). Body weights and fat content were significantly (p less than or equal to .05) reduced by feed restriction, with fat content only 39% of controls at 21 d off. Mortality averaged 0, 13.6, and 35.8% for rats fed 0, 5, or 10% MO, and 25, 15, 8.6, and 3.7% for rats feed restricted at 0, 15, 30, and 45%, respectively. Histopathology of the dead and moribund rats indicated that the clinical signs were not characteristic of PBB toxicity. In a second experiment, safflower oil at 3.5% or excess vitamins prevented the mortality and clinical signs associated with MO during withdrawal from 100 ppm PBBs. Based on these data and those in the literature, PBBs interfere with vitamin utilization.
给大鼠喂食浓度为0.1至100.0 ppm的多溴联苯（PBBs），持续14天，然后分别用0%、5%或10%的矿物油（MO）和/或0%、15%、30%或45%的饲料限制（FR）处理21天，以加速PBBs的清除。在第14天测定PBBs的体内负荷，并以对数-对数形式表示为Y = 0.91X + 2.179（r2 = 0.974），其中X = 饲料中PBBs浓度的对数（ppm），Y = PBBs体内负荷的对数（微克）。在撤药21天后，体内负荷用方程Y = 0.787X + 2.218（r2 = 0.95）表示。最有效的撤药处理是10%的MO + 45%的FR，体内负荷的降低与之前的体内负荷呈负相关（从0.1 ppm时的69%降至100 ppm时的23%）。饲料限制显著（p≤0.05）降低了体重和脂肪含量，在撤药21天时脂肪含量仅为对照组的39%。喂食0%、5%或10% MO的大鼠死亡率平均分别为0%、13.6%和35.8%，饲料限制分别为0%、15%、30%和45%的大鼠死亡率分别为25%、15%、8.6%和3.7%。死亡和濒死大鼠的组织病理学检查表明，临床症状并非PBBs毒性的特征。在第二项实验中，3.5%的红花油或过量的维生素可预防从100 ppm PBBs撤药期间与MO相关的死亡率和临床症状。基于这些数据和文献中的数据，PBBs会干扰维生素的利用。
