Randich Alan, Uzzell Tyler, Cannon Ronda, Ness Timothy J
Department of Psychology, University of Alabama, Birmingham, AL 35294, USA.
BMC Urol. 2006 Feb 9;6:2. doi: 10.1186/1471-2490-6-2.
Mycotic infections of the bladder produce pain and inflammatory changes. The present study examined the inflammatory and nociceptive effects of the yeast cell wall component, zymosan, when administered into the urinary bladder in order to characterize this form of bladder sensitization.
Parametric analyses of the time-course (0-48 hr) and concentration (0-2% solutions) variables associated with intravesical zymosan-induced bladder inflammation were performed in female rats. Plasma extravasation of Evan's Blue dye was used as a measure of tissue inflammation. Cardiovascular and visceromotor responses to urinary bladder distension were used as measures of nociception.
Zymosan-induced bladder inflammation, as indexed by plasma extravasation of Evan's Blue, was significantly greater in rats treated with either 1 or 2% solutions as compared to either 0.1 or 0.5% zymosan solutions. In time-course studies (1-48 hr post-treatment), 1% zymosan-induced inflammation progressively increased with time following administration, was greatest at 24 hr and began to normalize by 48 hr. In the studies of inflammation-induced changes in nociception, arterial blood pressure (ABP) and visceromotor responses to graded distension of the urinary bladder were significantly increased relative to controls 24 hr after zymosan administration.
These studies provide important time-course and solution concentration parameters for studies of zymosan-induced inflammation of the bladder and suggest utility of this model for the study of bladder-related pain.
膀胱真菌感染会产生疼痛和炎症变化。本研究检测了酵母细胞壁成分zymosan注入膀胱时的炎症和伤害感受作用，以明确这种膀胱致敏形式的特征。
对雌性大鼠进行与膀胱内zymosan诱导的膀胱炎症相关的时间进程（0 - 48小时）和浓度（0 - 2%溶液）变量的参数分析。伊文思蓝染料的血浆外渗用作组织炎症的指标。对膀胱扩张的心血管和内脏运动反应用作伤害感受的指标。
与0.1%或0.5%的zymosan溶液相比，用1%或2%溶液处理的大鼠中，以伊文思蓝血浆外渗为指标的zymosan诱导的膀胱炎症明显更严重。在时间进程研究（处理后1 - 48小时）中，1% zymosan诱导的炎症在给药后随时间逐渐增加，在24小时时最严重，并在48小时时开始恢复正常。在炎症诱导的伤害感受变化研究中，zymosan给药24小时后，相对于对照组，动脉血压（ABP）和对膀胱分级扩张的内脏运动反应显著增加。
这些研究为zymosan诱导的膀胱炎症研究提供了重要的时间进程和溶液浓度参数，并表明该模型在膀胱相关疼痛研究中的实用性。
