Sun Miao-Miao, Ma Jun, Sugiyama Hiromu, Ando Katsuhiko, Li Wen-Wen, Xu Qian-Ming, Liu Guo-Hua, Zhu Xing-Quan
College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province, 230036, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, China.
Parasitol Res. 2016 Oct;115(10):4013-20. doi: 10.1007/s00436-016-5171-6. Epub 2016 Jun 14.
Gnathostoma doloresi is one of the neglected pathogens causing gnathostomiasis. Although this zoonotic parasite leads to significant socioeconomic concerns globally, little is known of its genetics and systematics. In the present study, we sequenced and characterized the complete mitochondrial (mt) genomes of G. doloresi isolates from China and Japan. The lengths of the mt genomes of the G. doloresi China and Japan isolates are 13,809 and 13,812 bp, respectively. Both mt genomes encode 36 genes, including 12 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. The gene order, transcription direction, and genome content are identical with its congener G. spinigerum. Phylogenetic analyses based on concatenated amino acid sequences of 12 PCGs by Bayesian inference (BI) indicated that G. doloresi are closely related to G. spinigerum. Our data provide an invaluable resource for studying the molecular epidemiology, phylogenetics, and population genetics of Gnathostoma spp. and should have implications for further studies of the diagnosis, prevention, and control of gnathostomiasis in humans and animals.
多氏颚口线虫是引起颚口线虫病的被忽视病原体之一。尽管这种人畜共患寄生虫在全球范围内引发了重大的社会经济问题，但其遗传学和系统学却鲜为人知。在本研究中，我们对来自中国和日本的多氏颚口线虫分离株的完整线粒体（mt）基因组进行了测序和特征分析。中国和日本的多氏颚口线虫分离株的mt基因组长度分别为13,809和13,812 bp。两个mt基因组都编码36个基因，包括12个蛋白质编码基因（PCG）、2个核糖体RNA基因和22个转运RNA基因。基因顺序、转录方向和基因组内容与其同属的棘颚口线虫相同。基于12个PCG的串联氨基酸序列通过贝叶斯推断（BI）进行的系统发育分析表明，多氏颚口线虫与棘颚口线虫密切相关。我们的数据为研究颚口线虫属的分子流行病学、系统发育学和群体遗传学提供了宝贵资源，并且应该对进一步研究人类和动物颚口线虫病的诊断、预防和控制具有启示意义。
