Venter G J, Hill E, Pajor I T, Nevill E M
Veterinary Research Institute, Onderstepoort, Republic of South Africa.
Onderstepoort J Vet Res. 1991 Mar;58(1):5-9.
Culicoides spp. in the Lowveld of the northern Transvaal, Republic of South Africa, were fed bluetongue virus serotypes 3 and 6 and African horsesickness virus serotype 1 through latex and chicken skin membranes. After an incubation period of 10 days at 25-27 degrees C, the infection rate of C. imicola for bluetongue virus serotypes 3 and 6 was established at 31% and 24% respectively. No African horsesickness virus could be recovered. The membrane feeding technique and handling procedures proved to be suitable for field studies.
在南非共和国北德兰士瓦低地，通过乳胶膜和鸡皮膜让库蠓属昆虫吸食蓝舌病毒血清型3和6以及非洲马瘟病毒血清型1。在25 - 27摄氏度下经过10天的潜伏期后，已确定小库蠓对蓝舌病毒血清型3和6的感染率分别为31%和24%。未检测到非洲马瘟病毒。膜饲技术和处理程序被证明适用于野外研究。
