Venter G J, Mellor P S, Wright I, Paweska J T
ARC-Onderstepoort Veterinary Institute, Onderstepoort, South Africa.
Med Vet Entomol. 2007 Sep;21(3):239-47. doi: 10.1111/j.1365-2915.2007.00687.x.
Field-collected South African Culicoides (Diptera, Ceratopogonidae) were fed on sheep blood containing 16 live-attenuated vaccine strains of bluetongue virus (BTV) comprising serotypes -1, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, -14, -16 and -19. After 10 days extrinsic incubation at 23.5 degrees C, 11 and seven of the 16 BTV serotypes used were recovered from Culicoides (Avaritia) imicola Kieffer and Culicoides (A.) bolitinos Meiswinkel, respectively. One serotype was also recovered from Culicoides (Remmia) enderleini Cornet & Brunhes. Bluetongue virus recovery rates and the mean titres for most serotypes were significantly higher in C. bolitinos than in C. imicola. Significant differences were found in virus recovery rates from Culicoides species fed on blood containing similar or identical virus titres of different BTV serotypes. In addition, we demonstrated that a single passage of live-attenuated BTV-1, -2, -4, -9 and -16 through the insect vector, followed by passaging in insect cells, did not alter its infectivity for C. imicola and that the oral susceptibility of C. imicola to the attenuated vaccine strains of BTV-1, -4, -9 and -16 remained similar for at least three consecutive seasons.
用含有16种蓝舌病病毒（BTV）减毒活疫苗株的绵羊血喂养采自野外的南非库蠓（双翅目，蠓科），这些疫苗株包括血清型-1、-2、-3、-4、-5、-6、-7、-8、-9、-10、-11、-12、-13、-14、-16和-19。在23.5摄氏度下进行10天的外潜伏期后，分别从伊米库蠓（Avaritia）和博氏库蠓（A.）中回收了所使用的16种BTV血清型中的11种和7种。还从恩氏库蠓（Remmia）中回收了一种血清型。博氏库蠓中大多数血清型的蓝舌病病毒回收率和平均滴度显著高于伊米库蠓。在用含有不同BTV血清型相似或相同病毒滴度的血液喂养的库蠓种类中，病毒回收率存在显著差异。此外，我们证明，减毒活BTV-1、-2、-4、-9和-16在昆虫载体中传代一次，随后在昆虫细胞中传代，不会改变其对伊米库蠓的感染性，并且伊米库蠓对BTV-1、-4、-9和-16减毒疫苗株的口服易感性在至少三个连续季节中保持相似。
