Walter B A, Hawi A A, Zavos P M, Digenis G A
College of Pharmacy, University of Kentucky, Lexington 40536-0082.
Pharm Res. 1991 Mar;8(3):403-8. doi: 10.1023/a:1015818220894.
Selected oligomers representing the high, medium, and low molecular weight fractions of the spermicidal agent Nonoxynol-9 (N-9) were separated by HPLC. Nonoxynol-9 and the isolated fractions were formulated with polyvinylpyrrolidone (PVP) in order to increase their water solubility, particularly that of the insoluble low molecular weight fraction. The in vitro spermicidal activity of three molecular weight fractions were compared to that of N-9, using rabbit spermatozoa, at equimolar concentrations. Nonoxynol-9/PVP was far more effective in immobilizing the sperm than either N-9 alone or the separate fractions. The relative spermicidal activity of the oligomers was of the order middle molecular weight greater than high molecular weight greater than low molecular weight.
通过高效液相色谱法分离出了代表杀精剂壬苯醇醚-9（N-9）高、中、低分子量部分的特定低聚物。将壬苯醇醚-9和分离出的部分与聚乙烯吡咯烷酮（PVP）一起配制，以提高它们的水溶性，尤其是不溶性低分子量部分的水溶性。使用兔精子，在等摩尔浓度下比较了三种分子量部分与N-9的体外杀精活性。壬苯醇醚-9/聚乙烯吡咯烷酮在使精子失活方面比单独的N-9或单独的部分有效得多。低聚物的相对杀精活性顺序为中分子量大于高分子量大于低分子量。
