Mania-Pramanik Jayanti, Potdar Shobha, Kerkar Shilpa
National Institute for Research in Reproductive Health, Indian Council of Medical Research, Mumbai, India.
J Clin Lab Anal. 2006;20(1):8-14. doi: 10.1002/jcla.20092.
Important progress in the diagnosis of Chlamydia trachomatis (C. trachomatis) includes the development of nucleic acid amplification techniques such as polymerase chain reaction (PCR) and ligase chain reaction (LCR). Commercial kits are available, but they are costly, sporadic in availability, must be imported, and are economically beyond the reach of common people. To overcome this limitation, most research laboratories have standardized their in-house-developed PCR methods for diagnosing this infection. However, each laboratory has to spend a great deal of time and money to accomplish this. Published reports do not always elaborate the steps involved in standardizing a test so that it can immediately be reproduced in another setting. In the present study we attempted to elaborate the steps involved in standardizing a sensitive and specific PCR technique followed by hybridization with specific C. trachomatis probe to diagnose this infection in cervical, introital, and urine specimens, and used it to determine the infection rate in a clinical population.
沙眼衣原体（C. trachomatis）诊断方面的重要进展包括核酸扩增技术的发展，如聚合酶链反应（PCR）和连接酶链反应（LCR）。有商业化试剂盒可用，但它们成本高昂、供应零散、必须进口，普通民众在经济上难以承受。为克服这一限制，大多数研究实验室已将其自行研发的用于诊断该感染的PCR方法标准化。然而，每个实验室都必须花费大量时间和金钱来完成这一工作。已发表的报告并不总是详细阐述使一项检测标准化以便能在另一环境中立即重现的步骤。在本研究中，我们试图详细阐述使一种敏感且特异的PCR技术标准化的步骤，随后与特异的沙眼衣原体探针杂交以诊断宫颈、阴道口和尿液标本中的该感染，并将其用于确定临床人群中的感染率。
