固定在金表面的电操控单链和双链DNA的不同动力学行为。
Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface.
作者信息
Rant Ulrich, Arinaga Kenji, Tornow Marc, Kim Yong Woon, Netz Roland R, Fujita Shozo, Yokoyama Naoki, Abstreiter Gerhard
机构信息
Walter Schottky Institute, Technical University Munich, 85748 Garching, Germany.
出版信息
Biophys J. 2006 May 15;90(10):3666-71. doi: 10.1529/biophysj.105.078857. Epub 2006 Feb 10.
Abstract
We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.
摘要
我们报道了在电解质溶液中末端连接到金表面的单链和双链寡聚脱氧核苷酸的电操纵。通过时间分辨荧光测量研究了对交替的排斥性和吸引性电表面场的响应，分别揭示了柔性单链DNA和刚性双链DNA明显不同的动力学。流体动力学模拟解释了这一发现，并揭示了两种不同的动力学机制：刚性聚合物围绕锚定枢轴点旋转；另一方面，柔性聚合物逐段被拉到吸引表面上。
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