Fujimoto Akihisa, Onodera Hisashi, Mori Akira, Nagayama Satoshi, Yonenaga Yoshikuni, Tachibana Tsuyoshi
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
Anticancer Res. 2006 Jan-Feb;26(1A):59-69.
The concepts of vasculogenic mimicry and mosaic vessels have been proposed as novel modes of tumour neovascularisation. However, the presence and significance of these types of neovascularisation remain unclear.
ECV304 human bladder carcinoma cells were used to determine how tumour cells take part in tumour neovascularisation.
Subcutaneous ECV304 xenografts in mice showed various vessel types, including angiogenic vessels, tumour cell-related vessels and extracellular matrix networks. A tracer experiment demonstrated perfusion of beads in these structures. ECV304 cells, cultured on collagen I gels, formed tube networks with expressions of several endothelial-related markers. In coculture models of ECV304 cells and human umbilical vein endothelial cells, the two cells collaborated to form sprouts or networks.
ECV304 cells possess an endothelial character which confers the ability to mimic and collaborate with vascular endothelial cells and facilitates the acquisition of tumour microcirculation.
血管生成拟态和镶嵌血管的概念已被提出作为肿瘤新生血管形成的新模式。然而，这些类型的新生血管形成的存在及意义仍不明确。
采用ECV304人膀胱癌细胞来确定肿瘤细胞如何参与肿瘤新生血管形成。
小鼠皮下ECV304异种移植瘤显示出多种血管类型，包括血管生成性血管、肿瘤细胞相关血管和细胞外基质网络。示踪实验证明珠子可在这些结构中灌注。在I型胶原凝胶上培养的ECV304细胞形成了具有多种内皮相关标志物表达的管状网络。在ECV304细胞与人脐静脉内皮细胞的共培养模型中，两种细胞协同形成芽或网络。
ECV304细胞具有内皮特性，赋予其模仿血管内皮细胞并与之协作的能力，促进肿瘤微循环的形成。
