Eder Claudia, Falkner Erwin, Nehrer Stefan, Losert Udo M, Schoeffl Harald
BioMed-Society for the Promotion of Biomedical and Medical Technological Research in Upper Austria, Linz, Austria.
ALTEX. 2006;23(1):17-23.
Tissue engineering, defined as using a combination of cultured cells and biodegradable scaffolds to repair tissue damaged by injury or disease, represents a booming sector of biomedical research. Animal experimentation is routinely performed prior to clinical trials. The presented study tries to translate the aspect of the 3Rs to tissue engineering research: Cell culture protocols were adapted to antibiotic free and serum free conditions. Biomaterials (Bio-Gide and a collagen sponge prototype) were pre-tested using the HET-CAM assay. CAM-testing suggested a protocol change for application of the Bio-Gide scaffold and demonstrated unsuitable material properties of the collagen sponge. Application of 3R compliant protocols for tissue engineering research led to increased cell proliferation, higher synthesis of extracellular matrix molecules, reduced dedifferentiation and more information about the biomaterials at an early experimental stage. Tissue engineering research can therefore profit from the increased efforts to validate in vitro alternatives and supplements to animal testing.
组织工程学是指利用培养的细胞和可生物降解支架的组合来修复因损伤或疾病而受损的组织，它是生物医学研究中一个蓬勃发展的领域。在进行临床试验之前，通常会进行动物实验。本研究试图将3R原则应用于组织工程研究：调整细胞培养方案以适应无抗生素和无血清条件。使用鸡胚绒毛尿囊膜（HET-CAM）试验对生物材料（Bio-Gide和一种胶原海绵原型）进行了预测试。CAM测试表明Bio-Gide支架的应用方案需要改变，并证明了胶原海绵的材料性能不合适。在组织工程研究中应用符合3R原则的方案，可在早期实验阶段提高细胞增殖、增加细胞外基质分子的合成、减少去分化，并获得更多关于生物材料的信息。因此，组织工程研究可以从加大力度验证动物试验的体外替代方法和补充方法中受益。
