玄参提取物诱导人白血病细胞凋亡和 G2 /M 细胞周期阻滞。
Induction of apoptosis and G2 /M cell cycle arrest by Scrophularia striata in a human leukaemia cell line.
机构信息
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, 3419759811, Iran.
出版信息
Cell Prolif. 2013 Dec;46(6):637-43. doi: 10.1111/cpr.12074.
OBJECTIVES
Scrophularia striata Boiss (Scrophulariaceae) is a plant that grows in northeastern Iran; it has been used traditionally to treat various inflammatory disorders. This study was designed to investigate cytotoxic effects of S. striata extract, on the Jurkat human leukaemia cell line (T-cell leukaemia).
MATERIALS AND METHODS
Phytochemical assay by thin layer chromatography and 2, 2 diphenyl-1-picryl-hydrazyl were used to evaluate main compounds and antioxidant capacity of the plant extract, respectively. Its inhibitory effect on Jurkat cells was evaluated by MTT assay. In addition, cell cycle distribution and apoptotic cell death were evaluated by propidium iodide and annexin V-FITC/ propidium iodide staining.
RESULTS
These showed that the main components present in S. striata extract included flavonoids, phenolic compounds and phenyl propanoids. Treatment with extract was significantly cytotoxic to the tumour cell line. In addition, flow cytometry analysis indicated that S. striata extract induced cell cycle arrest in G2 /M phase and apoptosis of tumour cells.
CONCLUSIONS
Results of the study indicated that S. striata extract could inhibit leukaemia cell proliferation by inducing G2 /M phase arrest and apoptosis.
目的
Scrophularia striata Boiss（玄参科）是一种生长在伊朗东北部的植物，传统上用于治疗各种炎症性疾病。本研究旨在研究 Scrophularia striata 提取物对 Jurkat 人白血病细胞系（T 细胞白血病）的细胞毒性作用。
材料和方法
采用薄层色谱法和 2,2-二苯基-1-苦基肼评估植物提取物中的主要化合物和抗氧化能力。通过 MTT 测定法评估其对 Jurkat 细胞的抑制作用。此外，通过碘化丙啶和 Annexin V-FITC/碘化丙啶染色评估细胞周期分布和凋亡细胞死亡。
结果
结果表明，Scrophularia striata 提取物中的主要成分包括黄酮类、酚类化合物和苯丙素类。提取物对肿瘤细胞系具有显著的细胞毒性。此外，流式细胞术分析表明，Scrophularia striata 提取物诱导肿瘤细胞周期停滞在 G2/M 期并诱导细胞凋亡。
结论
研究结果表明，Scrophularia striata 提取物通过诱导 G2/M 期阻滞和细胞凋亡抑制白血病细胞增殖。
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