亚麻细胞培养物提取物的细胞毒性活性。

Cytotoxic activity of extracts from Linum cell cultures.

作者信息

Vasilev N P, Ionkova I

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Sofia 1000, Bulgaria.

出版信息

Fitoterapia. 2005 Jan;76(1):50-3. doi: 10.1016/j.fitote.2004.10.008.

DOI:10.1016/j.fitote.2004.10.008

PMID:15664462

Abstract

Callus and suspension cultures of Linum narbonense and Linum leonii were developed to study the production of lignans and their cytotoxic activity. Justicidin B was determined to be the main lignan. The maximal yield of justicidin B up to 2.22 mg/g of the cell dry weight was detected in the callus cultures of L. leonii, followed by the callus cultures of L. narbonense (1.57 mg/g dwt). The cytotoxicity of the obtained extracts was measured using the MTT-dye assay. L. narbonense and L. leonii both showed cytotoxic activity.

摘要

为了研究木脂素的产生及其细胞毒性活性，开发了纳博讷亚麻（Linum narbonense）和莱昂亚麻（Linum leonii）的愈伤组织和悬浮培养物。已确定正义霉素B是主要的木脂素。在莱昂亚麻的愈伤组织培养物中检测到正义霉素B的最大产量高达2.22毫克/克细胞干重，其次是纳博讷亚麻的愈伤组织培养物（1.57毫克/克干重）。使用MTT染料法测量所得提取物的细胞毒性。纳博讷亚麻和莱昂亚麻均显示出细胞毒性活性。