Vidal N, Mulanga C, Bazepeo S Edidi, Mwamba J Kasali, Tshimpaka J, Kashi M, Mama N, Valéa D, Delaporte E, Lepira F, Peeters M
UMR145, Laboratoire Retrovirus, IRD, BP 64501, 34394 Montpellier Cedex 1, France.
AIDS Res Hum Retroviruses. 2006 Feb;22(2):202-6. doi: 10.1089/aid.2006.22.202.
To study recombination and the natural polymorphism in pol of HIV-1 strains in the Democratic Republic of Congo (DRC) we sequenced the protease and RT genes for 70 HIV-1 strains previously characterized in the env V3-V5 region from a sentinel surveillance study in 2002. For 41 of the 70 (58.6%) strains, the same subtype/ CRF designations were observed in pol and env. Twenty-three (32.9%) of 70 pol sequences were complex recombinants involving two to five subtypes as well as fragments that could not be classified into any of the known subtypes. All subtypes were involved in recombination events. Unclassified (U) and env subtype H strains were very likely to be recombinant strains. Overall, many minor mutations were identified in the protease sequences. Although at the time of our study ARV use was not yet widespread in DRC, three strains were identified with one major mutation associated with drug resistance: L90M and M46L in protease and K103N in RT.
为了研究刚果民主共和国（DRC）HIV-1毒株pol基因中的重组和自然多态性，我们对2002年哨点监测研究中先前在env V3-V5区域鉴定的70株HIV-1毒株的蛋白酶和逆转录酶（RT）基因进行了测序。在70株毒株中，有41株（58.6%）在pol和env基因中观察到相同的亚型/循环重组型（CRF）分类。70条pol序列中有23条（32.9%）是复杂重组体，涉及两到五个亚型以及无法归类到任何已知亚型的片段。所有亚型都参与了重组事件。未分类（U）和env基因亚型H的毒株很可能是重组毒株。总体而言，在蛋白酶序列中鉴定出许多微小突变。尽管在我们研究时抗逆转录病毒药物在刚果民主共和国尚未广泛使用，但鉴定出三株带有一个与耐药性相关的主要突变的毒株：蛋白酶基因中的L90M和M46L以及RT基因中的K103N。
