细菌视紫红质光循环中中间光谱的先验解析：揭示L光谱的时间演化
A priori resolution of the intermediate spectra in the bacteriorhodopsin photocycle: the time evolution of the L spectrum revealed.
作者信息
Zimányi László, Saltiel Jack, Brown Leonid S, Lanyi Janos K
出版信息
J Phys Chem A. 2006 Feb 23;110(7):2318-21. doi: 10.1021/jp056874v.
Abstract
Resolution of the spectra of the intermediates in the photocycle of wild-type bacteriorhodopsin (BR) was achieved by singular value decomposition with exponential-fit-assisted self-modeling (SVD-EFASM) treatment of multichannel difference spectra measured at 5 degrees C during the course of the photocycle. New is the finding that two spectrally distinct L intermediates, L(1) and L(2), form sequentially. Our conclusion is that the photocycle is more complex than most published schemes. The dissection of the spectrally different L forms eliminates stoichiometric discrepancies usually appearing as systematically varying total intermediate concentrations before the onset of BR recovery. In addition, our analysis reveals that the red tails in the spectra of K and L(1) are more substantial than those of L(2) and BR. We suggest that these subtle differences in the shapes of the spectra reflect torsional and/or environmental differences in the retinyl chromophore.
摘要
通过对野生型细菌视紫红质（BR）光循环过程中在5摄氏度下测量的多通道差光谱进行奇异值分解与指数拟合辅助自建模（SVD - EFASM）处理，实现了对光循环中间体光谱的解析。新的发现是，两种光谱上不同的L中间体，L(1)和L(2)，是依次形成的。我们的结论是，光循环比大多数已发表的方案更为复杂。对光谱上不同的L形式的剖析消除了通常在BR恢复开始前作为总中间体浓度系统变化而出现的化学计量差异。此外，我们的分析表明，K和L(1)光谱中的红尾比L(2)和BR的更显著。我们认为，光谱形状的这些细微差异反映了视黄醛发色团的扭转和/或环境差异。
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