Sureda A, Batle J M, Tauler P, Ferrer M D, Tur J A, Pons A
Laboratori de Ciències de l'Activitat Física, Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Spain.
Eur J Clin Nutr. 2006 Jul;60(7):838-46. doi: 10.1038/sj.ejcn.1602388. Epub 2006 Feb 15.
We have investigated the influence of vitamin C diet supplementation on the antioxidant response and nitrite levels in lymphocytes and erythrocytes during diving apnea.
Seven male professional apnea divers participated in a double blind crossover study. Divers were randomly assigned to either vitamin C supplemented or placebo groups. The subjects did not take any other supplements than the ones provided for this study.
One group was supplemented with vitamin C capsules (1 g per day) for 7 days while the other group took a placebo composed of lactose. The usual dietary habits of participants were assessed using a self-reported 7-days 24-h recall before the day of the study. Blood samples were taken under basal conditions, immediately after diving apnea for 4 h and after 1 h of recovery.
Catalase activity increased in erythrocytes (23%) and superoxide dismutase increased in lymphocytes (35%) during the recovery only in the placebo group. Lymphocyte ascorbate levels increased in the supplemented group after diving (85%) and maintained high at recovery. Plasma nitrite levels increased about twofold in both groups during the recovery. Erythrocyte nitrite levels increased after diving (50%) and about twofold during the recovery in the supplemented group. Nitrite levels and iNOS levels in lymphocytes were higher in the placebo group than in the supplemented during the recovery. Erythrocyte carbonyl derivates were unchanged in all situations.
Vitamin C supplementation influenced the antioxidant response and NO handling in erythrocytes and lymphocytes to the oxidative stress induced by hypoxia-reoxygenation.
我们研究了在屏气潜水期间补充维生素C饮食对淋巴细胞和红细胞抗氧化反应及亚硝酸盐水平的影响。
七名男性职业屏气潜水员参与了一项双盲交叉研究。潜水员被随机分配到补充维生素C组或安慰剂组。受试者除了本研究提供的补充剂外未服用任何其他补充剂。
一组补充维生素C胶囊（每天1克），持续7天，而另一组服用由乳糖组成的安慰剂。在研究当天之前，使用自我报告的7天24小时回顾法评估参与者的日常饮食习惯。在基础条件下、屏气潜水4小时后以及恢复1小时后采集血样。
仅在安慰剂组中，恢复期间红细胞中的过氧化氢酶活性增加（23%），淋巴细胞中的超氧化物歧化酶增加（35%）。补充组潜水后淋巴细胞抗坏血酸水平增加（85%），恢复时保持高水平。两组恢复期间血浆亚硝酸盐水平均增加约两倍。补充组潜水后红细胞亚硝酸盐水平增加（50%），恢复期间增加约两倍。恢复期间，安慰剂组淋巴细胞中的亚硝酸盐水平和诱导型一氧化氮合酶水平高于补充组。所有情况下红细胞羰基衍生物均无变化。
补充维生素C影响红细胞和淋巴细胞对缺氧-复氧诱导的氧化应激的抗氧化反应和一氧化氮处理。
