Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, Germany.
Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany.
PLoS One. 2020 Aug 13;15(8):e0237673. doi: 10.1371/journal.pone.0237673. eCollection 2020.
This study investigated the acute changes in full spectrum differential blood cell count including reticulocytes and immature reticulocytes after a voluntary maximal dry apnea in non-elite divers. Aim of the present study is to obtain information on important regulatory compensation mechanisms and to provide insights into apneic regulatory processes.
Ten apnea divers performed a voluntary dry mean apnea time of 317 sec [SD ±111 sec]. Differential blood cell count including reticulocytes was measured before and immediately after a single maximal breath-hold. To evaluate kinetics, blood samples were also taken after 30 min and 4 h. Value distributions are presented with dot plots. P-values were calculated using a mixed linear model for time dependency. Four difference values were compared to baseline values with Dunnett's procedure.
Significant changes were found in red blood cell parameters for erythrocytes, red cell distribution width, hematocrit, hemoglobin, MCV, reticulocytes and immature reticulocytes, and in white blood cell parameters for leucocytes, lymphocytes, immature granulocytes, monocytes, basophile granulocytes, neutrophil granulocytes and eosinophil granulocytes and for thrombocytes.
Adaptive mechanisms regarding cell counts in elite apnea divers are not readily transferable to non-elite recreational sportspersons. Divers and physicians should be aware of the limited adaptive performance of humans in the case of extended apnea.
本研究调查了非精英潜水员自愿进行最大干屏气后全谱差异血细胞计数（包括网织红细胞和未成熟网织红细胞）的急性变化。本研究的目的是获得关于重要调节补偿机制的信息，并深入了解屏气调节过程。
10 名潜水员进行了一次自愿的干屏气平均时间为 317 秒[SD±111 秒]。在单次最大屏气前和立即后测量差异血细胞计数，包括网织红细胞。为了评估动力学，在 30 分钟和 4 小时后也采集了血液样本。值分布以点图表示。使用混合线性模型计算时间依赖性的 P 值。使用 Dunnett 程序将四个差值与基线值进行比较。
红细胞参数（红细胞、红细胞分布宽度、血细胞比容、血红蛋白、MCV、网织红细胞和未成熟网织红细胞）以及白细胞参数（白细胞、淋巴细胞、未成熟粒细胞、单核细胞、嗜碱性粒细胞、中性粒细胞和嗜酸性粒细胞）和血小板的显著变化。
关于精英潜水员细胞计数的适应机制不容易转移到非精英休闲运动者身上。潜水员和医生应该意识到在延长屏气的情况下，人类的适应能力有限。
