Nickell Stephan, Kofler Christine, Leis Andrew P, Baumeister Wolfgang
Max Planck Institute for Biochemistry, Department of Structural Biology, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Nat Rev Mol Cell Biol. 2006 Mar;7(3):225-30. doi: 10.1038/nrm1861.
Cryo-electron tomography is an emerging imaging technique that has unique potential for molecular cell biology. At the present resolution of 4-5 nm, large supramolecular structures can be studied in unperturbed cellular environments and, in the future, it will become possible to map molecular landscapes inside cells in a more comprehensive manner. 'Visual proteomics' aims to complement and extend mass-spectrometry-based inventories, and to provide a quantitative description of the macromolecular interactions that underlie cellular functions.
冷冻电子断层扫描是一种新兴的成像技术，在分子细胞生物学领域具有独特的潜力。在目前4-5纳米的分辨率下，可以在未受干扰的细胞环境中研究大型超分子结构，并且在未来，以更全面的方式绘制细胞内分子图谱将成为可能。“视觉蛋白质组学”旨在补充和扩展基于质谱的清单，并对构成细胞功能基础的大分子相互作用进行定量描述。
