Baumeister Wolfgang
Department of Structural Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
FEBS Lett. 2005 Feb 7;579(4):933-7. doi: 10.1016/j.febslet.2004.10.102.
Electron tomography can provide three-dimensional reconstructions of large pleomorphic structures at molecular resolution. While the principles of electron tomography have been known for decades, its use has gathered momentum only in recent years. Technological advances have made it possible to apply it to ice-embedded biological material (cryotomography), thereby ensuring a close-to-life preservation of the samples. In combination with advanced computational methods, such as molecular identification based on pattern recognition, it is a promising approach to comprehensively map macromolecular architecture inside organelles and cells and to visualize macromolecules at work in their natural environment.
电子断层扫描能够在分子分辨率下对大型多形结构进行三维重建。虽然电子断层扫描的原理已为人所知数十年，但直到近年来它的应用才开始蓬勃发展。技术进步使得将其应用于冰冻包埋的生物材料（冷冻断层扫描）成为可能，从而确保对样本进行接近真实状态的保存。结合先进的计算方法，如基于模式识别的分子鉴定，它是一种有前景的方法，可全面绘制细胞器和细胞内的大分子结构，并在其自然环境中可视化处于工作状态的大分子。
