Sharma B K, Luthra U K, Shah K V
Department of Immunology and Infectious Diseases, School of Hygiene andPublic Health, John Hopkins University, Baltimore, MD 21205.
Ann Biol Clin (Paris). 1991;49(2):93-7.
The polymerase chain reaction (PCR) technology was used to identify human papillomaviruses (HPV) in 52 paraffin embedded cervical tissues from Indian women with chronic cervicitis, different grades of cervical dysplasia and invasive cervical carcinoma. The tissues were screened for amplification of the cellular beta-globin gene as well as of HPVs. Sets of primers designed to amplify a portion of the E6 gene of HPV 6, 11, 16, 18, 31, 33 and 35 were employed. HPV 6, 16 and 31 were identified in 58% of 33 beta-globin positive tissues as compared to 16% of 19 beta-globin negative tissues. HPV 11, 18, 33 and 35 were not identified in any of the specimens. Double infection of HPV 16 and 31 was observed in one case of carcinoma in situ and one case of invasive carcinoma. HPV-16 was the predominant virus in HPV positive cases of higher grades of cervical dysplasia (severe dysplasia and carcinoma in situ) and cervical cancer.
采用聚合酶链反应（PCR）技术，对52例来自患有慢性宫颈炎、不同级别的宫颈发育异常以及浸润性宫颈癌的印度女性的石蜡包埋宫颈组织进行人乳头瘤病毒（HPV）鉴定。对这些组织进行细胞β-珠蛋白基因以及HPV的扩增筛选。采用了设计用于扩增HPV 6、11、16、18、31、33和35的E6基因部分片段的引物组。在33例β-珠蛋白阳性组织中，58%检测出HPV 6、16和31，而在19例β-珠蛋白阴性组织中，这一比例为16%。在任何标本中均未检测出HPV 11、18、33和35。在1例原位癌和1例浸润癌中观察到HPV 16和31的双重感染。在高级别宫颈发育异常（重度发育异常和原位癌）及宫颈癌的HPV阳性病例中，HPV-16是主要病毒。
