Burguete María C, Torregrosa Germán, Pérez-Asensio Fernando J, Castelló-Ruiz María, Salom Juan B, Gil José V, Alborch Enrique
Unidad de Circulación Cerebral Experimental, Centro de Investigación, Hospital Universitario La Fe, Ave. Campanar 21, 46009-Valencia, Spain.
Eur J Neurosci. 2006 Feb;23(3):703-10. doi: 10.1111/j.1460-9568.2006.04599.x.
As phytoestrogens are postulated as being neuroprotectants, we assessed the hypothesis that dietary isoflavone-type phytoestrogens are neuroprotective against ischemic stroke. Transient focal cerebral ischemia (90 min) was induced by middle cerebral artery occlusion (MCAO) following the intraluminal thread technique, both in rats fed with soy-based diet and in rats fed with isoflavone-free diet. Cerebro-cortical laser-Doppler flow (cortical perfusion, CP), arterial blood pressure, core temperature, PaO2, PaCO2, pH and glycemia were measured before, during and after MCAO. Neurological examination and infarct volume measurements were carried out 3 days after the ischemic insult. Dietary isoflavones (both glycosides and aglycones) were measured by high-performance liquid chromatography. Neither pre-ischemic, intra-ischemic nor post-ischemic CP values were significantly different between the soy-based diet and the isoflavone-free diet groups. Animals fed with the soy-based diet showed an infarct volume of 122 +/- 20.2 mm3 (19 +/- 3.3% of the whole ipsilateral hemisphere volume). In animals fed with the isoflavone-free diet the mean infarct volume was significantly higher, 191 +/- 26.7 mm3 (28 +/- 4.1%, P < 0.05). Neurological examination revealed significantly higher impairment in the isoflavone-free diet group compared with the soy-based diet group (3.3 +/- 0.5 vs. 1.9 +/- 0.5, P < 0.05). These results demonstrate that dietary isoflavones improve stroke outcome after transient focal cerebral ischemia in such a way that a higher dietary isoflavone content results in a lower infarct volume and a better neurological status.
由于植物雌激素被认为具有神经保护作用，我们评估了膳食异黄酮类植物雌激素对缺血性中风具有神经保护作用这一假说。采用腔内穿线技术，通过大脑中动脉闭塞（MCAO）诱导短暂性局灶性脑缺血（90分钟），实验对象包括喂食大豆基饮食的大鼠和喂食无大豆异黄酮饮食的大鼠。在MCAO之前、期间和之后，测量脑皮质激光多普勒血流（皮质灌注，CP）、动脉血压、核心体温、动脉血氧分压（PaO2）、动脉血二氧化碳分压（PaCO2）、pH值和血糖。在缺血性损伤3天后进行神经学检查和梗死体积测量。通过高效液相色谱法测量膳食异黄酮（糖苷和苷元）。大豆基饮食组和无大豆异黄酮饮食组之间，缺血前、缺血期间和缺血后的CP值均无显著差异。喂食大豆基饮食的动物梗死体积为122±20.2立方毫米（占同侧整个半球体积的19±3.3%）。喂食无大豆异黄酮饮食的动物平均梗死体积显著更高，为191±26.7立方毫米（28±4.1%，P<0.05）。神经学检查显示，无大豆异黄酮饮食组的损伤明显高于大豆基饮食组（3.3±0.5对1.9±0.5，P<0.05）。这些结果表明，膳食异黄酮可改善短暂性局灶性脑缺血后的中风预后，膳食异黄酮含量越高，梗死体积越小，神经状态越好。
