Boyle Michael D P, Hess Jennifer L, Nuara Anthony A, Buller R Mark
Department of Biology, Juniata College, Huntingdon, PA 16652, USA.
Methods. 2006 Apr;38(4):342-50. doi: 10.1016/j.ymeth.2005.11.011.
Cytokines are pivotal to a balanced innate or cell-mediated immune response, and can be indicative of disease progression and/or resolution. Methods to measure key cytokines rapidly with accuracy, precision, and sensitivity are consequently important. The current assay technologies, which are based on RT-PCR, immunoassays, or bioassays, are limited in their use in the clinic, in particular because of the long time (1-3 h) required to carry out the assays. An alternative, semi-quantitative approach described here, uses an immunological capture step and a mass spectral readout. The goal of the assay is speed rather than sensitivity or precision.
细胞因子对于平衡的先天性或细胞介导的免疫反应至关重要，并且可以指示疾病的进展和/或缓解。因此，能够快速、准确、精确且灵敏地测量关键细胞因子的方法非常重要。目前基于逆转录聚合酶链反应（RT-PCR）、免疫测定或生物测定的检测技术在临床应用中存在局限性，特别是因为进行这些检测需要较长时间（1 - 3小时）。本文描述的另一种半定量方法，采用免疫捕获步骤和质谱读出。该检测的目标是速度而非灵敏度或精密度。
