不同乙醇植物提取物对 L. 的植物化学分析及抗炎活性

Phytochemical Analysis and Anti-Inflammatory Activity of Different Ethanolic Phyto-Extracts of L.

机构信息

Department of Molecular and Translational Medicine, Division of Pharmacology, University of Brescia, 25123 Brescia, Italy.

Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy.

出版信息

Biomolecules. 2021 Jul 2;11(7):975. doi: 10.3390/biom11070975.

L. (AA) has shown for many centuries important therapeutic virtues associated with the presence of artemisinin (ART). The aim of this study was to identify and quantify ART and other secondary metabolites in ethanolic extracts of AA and evaluate the biological activity in the presence of an inflammatory stimulus. In this work, after the extraction of the aerial parts of AA with different concentrations of ethanol, ART was quantified by HPLC and HPLC-MS. In addition, anthocyanins, flavanols, flavanones, flavonols, lignans, low-molecular-weight phenolics, phenolic acids, stilbenes, and terpenes were identified and semi-quantitatively determined by UHPLC-QTOF-MS untargeted metabolomics. Finally, the viability of human neuroblastoma cells (SH-SY5Y) was evaluated in the presence of the different ethanolic extracts and in the presence of lipopolysaccharide (LPS). The results show that ART is more concentrated in AA samples extracted with 90% ethanol. Regarding the other metabolites, only the anthocyanins are more concentrated in the samples extracted with 90% ethanol. Finally, ART and all AA samples showed a protective action towards the pro-inflammatory stimulus of LPS. In particular, the anti-inflammatory effect of the leaf extract of AA with 90% ethanol was also confirmed at the molecular level since a reduction in TNF-α mRNA gene expression was observed in SH-SY5Y treated with LPS.

L.（AA）几个世纪以来一直显示出与青蒿素（ART）存在相关的重要治疗功效。本研究旨在鉴定和定量分析 AA 茎叶的醇提物中的 ART 和其他次生代谢产物，并评估在炎症刺激存在下的生物活性。在这项工作中，用不同浓度的乙醇提取 AA 的地上部分后，通过 HPLC 和 HPLC-MS 定量 ART。此外，通过 UHPLC-QTOF-MS 非靶向代谢组学鉴定并半定量测定了花色苷、黄烷醇、黄烷酮、黄酮醇、木脂素、低分子量酚类、酚酸、芪类和萜类。最后，评估了不同醇提物以及脂多糖（LPS）存在下人神经母细胞瘤细胞（SH-SY5Y）的活力。结果表明，ART 在 90%乙醇提取的 AA 样品中浓度更高。关于其他代谢物，只有花色苷在 90%乙醇提取的样品中浓度更高。最后，ART 和所有 AA 样品均对 LPS 的促炎刺激表现出保护作用。特别是，用 LPS 处理的 SH-SY5Y 中观察到 TNF-α mRNA 基因表达降低，这也证实了 90%乙醇提取的 AA 叶提取物的抗炎作用在分子水平上的作用。