Li L J, Yuan J F, Cai C A, Gu W G, Shi Z L
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
Arch Virol. 2006 Jul;151(7):1309-17. doi: 10.1007/s00705-005-0719-2. Epub 2006 Feb 20.
White spot syndrome virus (WSSV) is a devastating viral pathogen of cultured shrimp worldwide. Previous studies have shown that the intact virion consists of at least 39 structural proteins and, among them, six were identified as envelope proteins involved in the virus infection. In this paper, the structural proteins VP36A, VP36B and VP31 (J Virol 2004; 78: 11360-11370), containing the RGD motif, were expressed in Escherichia coli and used to produce specific antibodies. Western blot confirmed that VP36A is a newly reported envelope protein. A neutralization assay with these three antibodies demonstrated that VP36A, VP36B and VP31 could significantly delay the initial infection of crayfish, but mortality still reached 100% at day 11 post-injection. However, a neutralization assay with the combination of antibodies against different envelope proteins showed that a combination of VP36B and VP31 antibodies could strongly inhibit WSSV infection in crayfish. These results revealed that multiple envelope proteins are involved in WSSV infection in crayfish and that VP36B and VP31 play a key role during this process.
白斑综合征病毒（WSSV）是全球养殖虾类中一种极具破坏性的病毒病原体。先前的研究表明，完整的病毒粒子至少由39种结构蛋白组成，其中六种被鉴定为参与病毒感染的包膜蛋白。在本文中，含有RGD基序的结构蛋白VP36A、VP36B和VP31（《病毒学杂志》2004年；78：11360 - 11370）在大肠杆菌中表达并用于制备特异性抗体。蛋白质免疫印迹法证实VP36A是一种新报道的包膜蛋白。用这三种抗体进行的中和试验表明，VP36A、VP36B和VP31可显著延迟小龙虾的初始感染，但注射后第11天死亡率仍达100%。然而，用针对不同包膜蛋白的抗体组合进行的中和试验表明，VP36B和VP31抗体的组合可强烈抑制小龙虾中的WSSV感染。这些结果表明，多种包膜蛋白参与小龙虾中的WSSV感染，并且VP36B和VP31在此过程中起关键作用。
