Prenzel Klaus L, Warnecke-Eberz Ute, Brabender Jan, Baldus Stephan E, Bollschweiler Elfriede, Gutschow Christian A, Drebber Uta, Hoelscher Arnulf H, Schneider Paul M
Department of Visceral and Vascular Surgery, University of Cologne, Joseph-Stelzmann-Str. 9, 50931 Cologne, Germany.
World J Gastroenterol. 2006 Jan 21;12(3):437-42. doi: 10.3748/wjg.v12.i3.437.
We examined quantitative mRNA expression of growth factor receptors (c-erbB-1, c-erbB-2) and the anti-apoptosis gene survivin known to be regulated in pancreatic adenocarcinomas and compared the expression pattern with that in carcinomas of the papilla of Vater.
Quantitative real-time reverse transcriptase-PCR (QRT-PCR, Taqman) was performed to analyze mRNA expression levels of c-erbB-1, c-erbB-2 and survivin in normal and corresponding tumor samples of 31 pancreatic adenocarcinomas and 8 cancers of the papilla of Vater.
The overall median mRNA expression of survivin was significantly increased in both adenocarcinoma of the pancreas (P<0.01) and papilla of Vater (P<0.008) compared with uninvolved normal control tissue. In pancreatic cancer, expression of c-erbB-1 was significantly decreased compared with the normal pancreatic tissue (P<0.03), whereas in the cancer of the papilla of Vater expression of c-erbB-2 was significantly downregulated (P<0.05) compared with the paired normal samples. Gene expression was not associated with tumor stage, differentiation or prognosis.
The common anti-apoptosis gene survivin is overexpressed both in the cancer of the papilla of Vater and pancreas. In contrast, the growth factor receptor genes c-erbB-1 and c-erbB-2 are differentially regulated in both tumor entities adding further evidence that pancreatic cancer is biologically different from the cancer of papilla of Vater.
我们检测了已知在胰腺腺癌中受调控的生长因子受体（c-erbB-1、c-erbB-2）和抗凋亡基因survivin的定量mRNA表达，并将其表达模式与 Vater 壶腹癌的表达模式进行比较。
采用定量实时逆转录聚合酶链反应（QRT-PCR，Taqman 法）分析 31 例胰腺腺癌和 8 例 Vater 壶腹癌的正常及相应肿瘤样本中 c-erbB-1、c-erbB-2 和 survivin 的 mRNA 表达水平。
与未受累的正常对照组织相比，survivin 的总体中位 mRNA 表达在胰腺腺癌（P<0.01）和 Vater 壶腹癌（P<0.008）中均显著升高。在胰腺癌中，与正常胰腺组织相比，c-erbB-1 的表达显著降低（P<0.03），而在 Vater 壶腹癌中，与配对的正常样本相比，c-erbB-2 的表达显著下调（P<0.05）。基因表达与肿瘤分期、分化或预后无关。
常见的抗凋亡基因 survivin 在 Vater 壶腹癌和胰腺癌中均过度表达。相比之下，生长因子受体基因 c-erbB-1 和 c-erbB-2 在这两种肿瘤实体中受到不同的调控，这进一步证明胰腺癌在生物学上与 Vater 壶腹癌不同。
