猪自然感染或实验感染后对猪布鲁氏菌和小肠结肠炎耶尔森氏菌O:9血清型血清学反应的鉴别
Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O:9 after natural or experimental infection in pigs.
作者信息
Jungersen G, Sørensen V, Giese S B, Stack J A, Riber U
机构信息
Department of Veterinary Diagnostics and Research, Danish Institute of Food and Veterinary Research, Copenhagen, Denmark.
出版信息
Epidemiol Infect. 2006 Apr;134(2):347-57. doi: 10.1017/S095026880500511X.
Abstract
False-positive serological reactions (FPSR) due to infections with Yersinia enterocolitica serotype Oratio9 (YeOratio9) are a problem in tests for brucellosis. In the present study, FPSR in classical and novel tests for brucellosis following experimental infections of pigs with YeOratio9 were compared with responses of B. suis biovar 2-inoculated pigs. FPSR were limited to 2-9 weeks post-YeOratio9 inoculation, while B. suis-infected pigs were test-positive throughout the 21-week period of investigation. Although YeOratio9-inoculated pigs exhibited FPSR in Brucella tests for a limited period of time, the serological responses in a YeOratio9-purified O-antigen indirect ELISA did not decrease accordingly. Analysis of available cross-sectional serum samples from pig herds naturally infected with YeOratio9 or B. suis biovar 2 confirmed that the observed difference in the duration of the serological responses between the two infections could be used to discriminate between herds infected with B. suis biovar 2 and YeOratio9.
摘要
由小肠结肠炎耶尔森菌O:9血清型（YeO:9）感染引起的假阳性血清学反应（FPSR）是布鲁氏菌病检测中的一个问题。在本研究中，将猪经YeO:9实验感染后在经典和新型布鲁氏菌病检测中的FPSR与接种猪布鲁氏菌生物变种2的猪的反应进行了比较。FPSR仅限于YeO:9接种后2至9周，而感染猪布鲁氏菌的猪在整个21周的调查期内检测均为阳性。尽管接种YeO:9的猪在布鲁氏菌检测中在有限时间内出现FPSR，但YeO:9纯化O抗原间接ELISA中的血清学反应并未相应降低。对自然感染YeO:9或猪布鲁氏菌生物变种2的猪群现有横断面血清样本的分析证实，观察到的两种感染血清学反应持续时间的差异可用于区分感染猪布鲁氏菌生物变种2和YeO:9的猪群。
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