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酶联免疫吸附测定法,用于区分感染布鲁氏菌属的动物与感染小肠结肠炎耶尔森氏菌O9的动物的抗体反应。

Enzyme-linked immunosorbent assay to differentiate the antibody responses of animals infected with Brucella species from those of animals infected with Yersinia enterocolitica O9.

作者信息

Erdenebaatar Janchivdorj, Bayarsaikhan Balgan, Watarai Masahisa, Makino Sou-ichi, Shirahata Toshikazu

机构信息

Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

出版信息

Clin Diagn Lab Immunol. 2003 Jul;10(4):710-4. doi: 10.1128/cdli.10.4.710-714.2003.

Abstract

Enzyme-linked immunosorbent assays using antigens extracted from Brucella abortus with n-lauroylsarcosine differentiated natural Brucella-infected animals from Brucella-vaccinated or Yersinia enterocolitica O9-infected animals. A field trial in Mongolia showed cattle, sheep, goat, reindeer, camel, and human sera without infection could be distinguished from Brucella-infected animals by conventional serological tests.

摘要

使用从流产布鲁氏菌中提取的抗原与月桂酰肌氨酸进行的酶联免疫吸附测定,可区分自然感染布鲁氏菌的动物与接种布鲁氏菌疫苗或感染小肠结肠炎耶尔森氏菌O9的动物。在蒙古进行的一项现场试验表明,通过传统血清学检测,可以将未感染的牛、羊、山羊、驯鹿、骆驼和人类血清与感染布鲁氏菌的动物区分开来。

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