用于检测家畜布鲁氏菌感染抗体的竞争性酶联免疫吸附测定法的评估
Evaluation of competitive ELISA for detection of antibodies to Brucella infection in domestic animals.
作者信息
Perrett Lorraine L, McGiven John A, Brew Simon D, Stack Judy A
机构信息
Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency, Woodham Lane, Weybridge, Surrey, KT15 3NB, United Kingdom.
出版信息
Croat Med J. 2010 Aug;51(4):314-9. doi: 10.3325/cmj.2010.51.314.
Abstract
AIM
To evaluate competitive enzyme-linked immunosorbent assay (cELISA) for its suitability as an additional serological test for the diagnosis of animal brucellosis.
METHODS
cELISA, which was developed at the Veterinary Laboratories Agency, has been evaluated for its accuracy and suitability as an additional serological test for the diagnosis of animal brucellosis. Samples from naturally and experimentally infected animals and those from Brucella-free flocks and herds were tested.
RESULTS
Data obtained since 1991 were analyzed from routine surveillance, animals experimentally infected with Brucella, and stored sera to validate cELISA for the detection of antibodies to Brucella in cows, small ruminants, and pigs. The sensitivity of the test ranged from 92.31% to 100%, in comparison with 77.14% to 100% for the complement fixation test (CFT). Specificities for cELISA, indirect enzyme-linked immunosorbent assay, and CFT were greater than 90%.
CONCLUSION
cELISA can be used on a variety of animal species, and an added advantage is its suitability for use on poor-quality samples such as those affected by hemolysis.
摘要
目的
评估竞争酶联免疫吸附测定法（cELISA）作为动物布鲁氏菌病诊断的附加血清学检测方法的适用性。
方法
对由兽医实验室机构研发的cELISA作为动物布鲁氏菌病诊断的附加血清学检测方法的准确性和适用性进行评估。对来自自然感染和实验感染动物的样本以及来自无布鲁氏菌羊群和牛群的样本进行检测。
结果
分析了自1991年以来从常规监测、实验感染布鲁氏菌的动物以及储存血清中获得的数据，以验证cELISA在检测奶牛、小反刍动物和猪中布鲁氏菌抗体方面的有效性。该检测方法的灵敏度范围为92.31%至100%，相比之下，补体结合试验（CFT）的灵敏度为77.14%至100%。cELISA、间接酶联免疫吸附测定法和CFT的特异性均大于90%。
结论
cELISA可用于多种动物物种，其另一个优势是适用于诸如受溶血影响的劣质样本。
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