Clark I M, Powell L K, Wright J K, Cawston T E
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.
Matrix. 1991 Apr;11(2):76-85. doi: 10.1016/s0934-8832(11)80211-8.
Both polyclonal and monoclonal antibodies have been raised against purified human fibroblast tissue inhibitor of metalloproteinases (TIMP) and characterised. Combinations of antibodies were tested for their suitability in a double antibody sandwich ELISA to measure TIMP. Two combinations were applicable to the immunoassay: (i) a monoclonal capture antibody with a polyclonal detecting antibody; (ii) two monoclonal antibodies. The assay range was (i) 2-50 ng/ml and (ii) 5-50 ng/ml of human TIMP. The levels of TIMP in several human body fluids were measured using assay (ii), and values in ng/ml with standard deviations (n-1) obtained as follows: serum, 299 +/- 62; plasma, 109 +/- 35; amniotic fluid, 724 +/- 62; cerebrospinal fluid, 144 +/- 156; saliva, 209 +/- 103.
已经制备了针对纯化的人成纤维细胞金属蛋白酶组织抑制剂（TIMP）的多克隆抗体和单克隆抗体，并对其进行了表征。测试了抗体组合在双抗体夹心ELISA中测量TIMP的适用性。有两种组合适用于免疫测定：（i）一种单克隆捕获抗体与一种多克隆检测抗体；（ii）两种单克隆抗体。测定范围为（i）人TIMP 2-50 ng/ml和（ii）5-50 ng/ml。使用测定法（ii）测量了几种人体体液中的TIMP水平，以ng/ml为单位的值及其标准差（n-1）如下：血清，299±62；血浆，109±35；羊水，724±62；脑脊液，144±156；唾液，209±103。
