Zhang Qingjun, Li Xuepei, Zhu Li
Department of Otorhinolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Beifang College, Zhangjiakou, 075000, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Dec;19(23):1057-8, 1060.
To study the effect of fungi in the pathogenesis of nasal polyps and chronic rhinosinusitis (CRS).
This study were divided two groups, the first was the experimental group: Nasal mucus membrane and nasal polyps were collected from patients with CRS (with and without polyps), and the second was the control group, nasal mucus were collected from OHc. Fungal-specific PCR were performed on each sample. The anther was used 0.9% sol sodium chloride to wash the autopsy tissues 5 times, and then PCR analysis was performed. chi-squared analysis was used to test for statistical difference between these groups.
PCR technique detected fungal DNA were 89.2% and 89.4% in nasal polyps and nasal mucous membrane respectively, and 66.7% in normal control subjective. There were statistically significant difference in these groups. After using 0.9% sol sodium chloride to wash the autopsy tissues, the fungi's positive rate was dropped.
Fungi was important in the pathogenesis of nasal polyps and CRS.
研究真菌在鼻息肉和慢性鼻 - 鼻窦炎（CRS）发病机制中的作用。
本研究分为两组，第一组为实验组：从CRS患者（有息肉和无息肉）中采集鼻黏膜和鼻息肉，第二组为对照组，从正常健康对照者中采集鼻黏液。对每个样本进行真菌特异性PCR检测。用0.9%氯化钠溶液冲洗尸检组织5次，然后进行PCR分析。采用卡方分析检验这些组之间的统计学差异。
PCR技术检测到鼻息肉和鼻黏膜中真菌DNA的比例分别为89.2%和89.4%，正常对照者中为66.7%。这些组之间存在统计学显著差异。用0.9%氯化钠溶液冲洗尸检组织后，真菌阳性率下降。
真菌在鼻息肉和CRS的发病机制中起重要作用。
