Saikia Sanjay, Parker Emily J, Koulman Albert, Scott Barry
Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North 5301, New Zealand.
FEBS Lett. 2006 Mar 6;580(6):1625-30. doi: 10.1016/j.febslet.2006.02.008. Epub 2006 Feb 17.
Paspaline belongs to a large, structurally and functionally diverse group of indole-diterpenes synthesized by filamentous fungi. However, the identity of the gene products required for the biosynthesis of paspaline, a key intermediate for the synthesis of paxilline and other indole-diterpenes, is not known. Transfer of constructs containing different pax gene combinations into a paxilline negative deletion derivative of Penicillium paxilli demonstrated that just four proteins, PaxG, a geranylgeranyl diphosphate synthase, PaxM, a FAD-dependent monooxygenase, PaxB, a putative membrane protein, and PaxC, a prenyl transferase, are required for the biosynthesis of paspaline.
巴斯帕灵属于丝状真菌合成的一大类结构和功能多样的吲哚二萜。然而，作为合成鬼笔环肽和其他吲哚二萜的关键中间体，巴斯帕灵生物合成所需基因产物的身份尚不清楚。将含有不同pax基因组合的构建体导入产紫青霉鬼笔环肽阴性缺失衍生物中，结果表明，巴斯帕灵的生物合成仅需要四种蛋白质，即香叶基香叶基二磷酸合酶PaxG、FAD依赖性单加氧酶PaxM、推定的膜蛋白PaxB和异戊烯基转移酶PaxC。
